广西水稻产区稻-菜轮作田水稻根结线虫病发生情况调查及病原鉴定  

Survey and Pathgen Identification of Rice Root Knot Nematode Disease in Rice-Vegetable Rotation Fields in Guangxi

作　　者：黄金玲[1] 覃丽萍[1] 刘志明[1] 刘峥嵘 李红芳 陆秀红[1] HUANG Jinling;QIN Liping;LIU Zhiming;LIU Zhengrong;LI Hongfang;LU Xiuhong(Plant Protection Research Institute,Guangxi Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning 530007;College of Agriculture,Guangxi University,Nanning 530005)

机构地区：[1]广西壮族自治区农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室,南宁530007 [2]广西大学农学院,南宁530005

出　　处：《中国农学通报》2024年第12期126-133,共8页Chinese Agricultural Science Bulletin

基　　金：广西科技重大专项“广西区域农业高值化发展关键技术研究与应用”(桂科AA22036001);广西农业科学院科技发展基金“植物线虫研究”(桂农科2021YT062);“根结线虫的快速分子检测技术研究”(桂农科2021ZX24)。

摘　　要：为了明确广西水稻产区稻-菜轮作田根结线虫发生情况及其病原分类地位,在广西14个市27个乡(镇)的171块水稻产区稻-菜轮作田进行了采样调查,并进行致病线虫分离,根据2龄幼虫形态、雌虫会阴花纹特征等,结合核糖体ITS区和28S rDNA D2D3区序列分析方法对病原线虫种类进行鉴定。结果表明,共有64个田块发生根结线虫为害,水稻种植季根结线虫检出率为37.4%,蔬菜轮作季根结线虫检出率为25.7%。广西水稻根结线虫病病原为拟禾本科根结线虫Meloidogyne graminicola。说明拟禾本科根结线虫病在广西稻-菜轮作田发生已经相当普遍,应当引起重视。In order to investigate the occurrence of root knot nematode disease in rice-vegetable rotation fields of Guangxi,and clarify the pathogen species,a sampling survey on the occurrence of rice root-knot nematode disease was carried out in 171 rice-vegetable rotation fields in 27 townships of 14 cities in Guangxi.Nematodes were isolated from samples,and the species was identified by observing the morphological characteristics of second instar larvae and perineal pattern of females,and analyzing ribosomal ITS region and 28S rDNA D2D3 region sequences.The results showed that 64 fields of them were affected with root-knot nematodes,with a detection rate of 37.4%during the rice-growing season and 25.7%during the vegetable rotation.The species of root-knot nematode on rice in Guangxi was confirmed as Meloidogyne graminicola.It indicated that the disease caused by M.graminicola had occurred widely in rice-vegetable rotation fields in Guangxi,and more attention should be paid.

关 键 词：广西 稻-菜轮作 水稻根结线虫 调查 鉴定 

分 类 号：S432.45[农业科学—植物病理学]