影响糯稻直链淀粉含量的QTL及效应分析  

QTL and effect analysis affecting amylose content in glutinous rice

作　　者：史夏蕾 张玉婷 赵锦祥 肖长春 王泓超 梁莹莹 程祖锌[1] 林荔辉[1] SHI Xia-lei;ZHANG Yu-ting;ZHAO Jin-xiang;XIAO Chang-chun;WANG Hong-chao;LIANG Ying-ying;CHENG Zu-xin;LIN Li-hui(Key Laboratory of Crop Genetic Breeding and Comprehensive Utilization,Ministry of Education,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Sanming Academy of Agricultural Sciences,Sanming,Fujian 365509,China)

机构地区：[1]福建农林大学作物遗传育种与综合利用教育部重点实验室,福州350002 [2]三明市农业科学研究院,福建三明365509

出　　处：《西南农业学报》2024年第3期481-489,共9页Southwest China Journal of Agricultural Sciences

基　　金：福建省科技计划高校产学研合作项目(2022N5011);福建省(山区)作物遗传改良与创新利用重点实验室开放课题(2022SKF02)。

摘　　要：【目的】直链淀粉含量是影响糯稻品质的关键性状,旨在剖析其遗传基础对稻米品质改良的重要意义。【方法】构建‘品糯R191/金贵丝苗//金贵丝苗///金贵丝苗’的BC_(2)F_(4)和BC_(2)F_(5)回交遗传世代群体,在携带wxwx基因的遗传背景下,采用集团分离分析法,利用GSR40K水稻基因芯片对双亲和高、低池群体进行SNP分型,并根据分析结果锚定影响稻米直链淀粉含量的候选基因。【结果】定位到控制直链淀粉含量的2个候选基因区段,位于5号染色体0.04~0.37 Mb和12号染色体17.53~24.09 Mb。从12号染色体候选区间中筛选出14对具有多态性的SSR标记和SNP标记,利用QTL IciMapping软件构建遗传连锁图,在3种环境及不同世代下,采用完备区间作图法(Inclusive composite interval mapping,ICIM)进行QTL定位,在N21774~A24633区间检测到影响直链淀粉含量的QTL qAC12,区间距离为3.46 Mb,对直链淀粉含量贡献率均值为14.10%,加性效应值为-0.11。对5号染色体末端的候选QTL分析上,通过预测区间标记ZcI5_167的基因分型结果,并结合表型估算,该QTL qAC5对直链淀粉含量表型贡献率为9.83%,加性效应值为0.156。【结论】稻米直链淀粉含量不仅受Wx基因调控,还与其他QTLs基因密切相关。挖掘并分析其遗传基础,对水稻新品种品质育种具有重要意义。本研究定位到12号和5号染色体上影响糯稻直链淀粉含量的微效QTL,有助于糯稻直链淀粉含量的分子标记辅助选择,对降低糯稻的直链淀粉含量,提高糯稻稻米品质具有较好的实用价值。【Objective】Amylose content is a key trait affecting the quality of glutinous rice.The present paper aimed to analyze the important significance of its genetic basis for rice quality improvement.【Method】In the study,we constructed the BC_(2)F_(4) and BC_(2)F_(5) backcross genetic generation populations of‘Pinnuo R191/Jinguisimiao//Jinguisimiao///Jinguisimiao’in a genetic background carrying the wxwx gene,and used the group segregation analysis method to SNP-type both parents and the high and low pooled populations using the GSR40K rice Gene-Chip,candidate genes affecting amylose content in rice were anchored according to the analysis results.【Result】Two candidate gene segments controlling amylose content were located on chromosome 5 from 0.04 to 0.37 Mb and chromosome 12 from 17.53 to 24.09 Mb.14 pairs of polymorphic SSR markers and SNP markers were screened from the candidate intervals of chromosome 12,and the genetic linkage map was constructed by using QTL IciMapping software.By using Inclusive Composite Interval Mapping(ICIM),QTL qAC12 affecting amylose content was detected in the interval of N21774-A24633 with an interval distance of 3.46 Mb.The mean contribution rate to amylose content was 14.10%,and the additive effect value was-0.11.For the analysis of candidate QTL at the end of chromosome 5,based on the genotyping results of the predicted interval marker ZcI5_167,combined with the phenotype estimation,the contribution rate of QTL qAC5 to the phenotype of amylose content was 9.83%,and the additive effect value was 0.156.【Conlusion】The amylose content of rice is not only regulated by Wx gene,but also closely related to other QTLs genes.It is of great significance for the breeding of new varieties of high quality rice to excavate and analyze its genetic basis.In the study,the micropotent QTL on chromosome 12 and chromosome 5 affecting amylose content of glutinous rice is identified,which can be helpful for molecular marker-assisted breeding of amylose content of glutinous rice,and has go

关 键 词：糯稻 直链淀粉含量(AC) QTL 效应分析 

分 类 号：S511[农业科学—作物学]