胎盘组织中自噬相关分子标志物预测产妇子痫前期合并胎儿生长受限的应用价值  

The application value of autophagy related molecular markers in placental tissue for predicting preeclampsia combined with fetal growth restriction in pregnant women

作　　者：李铁平 黎丽[2] 唐玲丽 陈秋玲[2] 张满云[2] 施隽 杨琼娇[2] 唐雅兵 Li Tieping;Li Li;Tang Lingli;Chen Qiuling;Zhang Manyun;Shi Jun;Yang Qiongjiao;Tang Yabing(Department of Obstetrics,University of South China Affiliated Maternal and Child Health Care Hospital(Hunan Province Maternal and Child Health Care Hospital),Changsha 410008,China;Department of Obstetrics,Changsha Hospital for Maternal and Child Health Care,Changsha 410007,China)

机构地区：[1]南华大学附属妇幼保健院(湖南省妇幼保健院)产科,长沙410008 [2]长沙市妇幼保健院产科,长沙410007

出　　处：《中国医师杂志》2024年第4期533-537,542,共6页Journal of Chinese Physician

基　　金：湖南省卫健委课题(B202302076420);长沙市妇幼保健院院级课题(2022YJ06)。

摘　　要：目的分析胎盘组织中自噬相关分子标志物预测产妇子痫前期合并胎儿生长受限的应用价值。方法收集长沙市妇幼保健院2021年1月至2023年8月收治的46例孕妇,采用横断面研究方法,根据妊娠结局分为正常分娩组(对照组,23例)和子痫前期合并胎儿生长受限组(观察组,23例),分别采用透射电镜观察两组胎盘组织中滋养细胞内自噬泡的发生情况;通过免疫组织化学法、实时荧光定量聚合酶链式反应(qRT-PCR)、蛋白质印迹法(Western blot)检测两组胎盘组织中Beclin-1、LC3和p62的表达情况。结果透射电镜结果显示,对照组和观察组的胎盘滋养层细胞中均可观察到自噬泡的存在,但观察组胎盘组织滋养细胞内自噬泡数量多于对照组(P<0.05)。免疫组化结果显示,观察组胎盘组织切片中Beclin-1和LC3的显色度高于对照组(均P<0.001),而p62的显色度低于对照组(P<0.001)。qRT-PCR和Western blot结果显示,观察组Beclin-1和LC3 mRNA和蛋白表达水平明显高于对照组(均P<0.001),而p62 mRNA和蛋白表达水平则低于对照组(均P<0.001)。结论子痫前期合并胎儿生长受限者胎盘组织滋养细胞自噬活性增强,提示自噬水平的升高与子痫前期合并胎儿生长受限结局的发生有密切关联,过度的自噬可能参与了子痫前期合并胎儿生长受限结局的发生。Objective To analyze the application value of autophagy related molecular markers in placenta tissue in predicting fetal growth restriction in pregnant women with preeclampsia.Methods A total of 46 pregnant women admitted to Changsha Hospital for Maternal and Child Health Care from January 2021 to August 2023 were collected.A cross-sectional study was conducted,and they were divided into a normal delivery group(control group,23 cases)and an observation group with preeclampsia combined with fetal growth restriction(observation group,23 cases)based on pregnancy outcomes.Transmission electron microscopy was used to observe the occurrence of autophagic vesicles in trophoblast cells in the placental tissue of both groups;The expression of Beclin-1,LC3,and P62 in placental tissues of two groups was detected by immunohistochemistry,real-time fluorescence quantitative polymerase chain reaction(qRT-PCR),and Western blot.Results The transmission electron microscopy results showed that the presence of autophagic vesicles could be observed in the placental trophoblast cells of both the control group and the observation group,but the number of autophagic vesicles in the trophoblast cells of the observation group was higher than that in the control group(P<0.05).The immunohistochemical results showed that the colorimetric values of Beclin-1 and LC3 in the placental tissue sections of the observation group were higher than those of the control group(all P<0.001),while the colorimetric values of p62 were lower than those of the control group(P<0.001).The qRT-PCR and Western blot results showed that the mRNA and protein expression levels of Beclin-1 and LC3 in the observation group were significantly higher than those in the control group(all P<0.001),while the expression levels of p62 mRNA and protein were lower than those in the control group(all P<0.001).Conclusions The autophagy activity of placental tissue trophoblasts in patients with preeclampsia combined with fetal growth restriction is enhanced,indicating a close correla

关 键 词：自噬相关蛋白质 胎盘 子痫前期 胎儿生长受限 

分 类 号：R714.244[医药卫生—妇产科学] R714.5[医药卫生—临床医学]