基于纳米电极的单细胞内生物分子电化学分析  

Electrochemical Analysis of Intracellular Molecules at Single Cells Based on Nanoelectrodes

作　　者：刘康 潘荣容 江德臣 LIU Kang;PAN Rongrong;JIANG Dechen(State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210092,China)

机构地区：[1]南京大学化学化工学院,生命分析化学国家重点实验室,南京210092

出　　处：《高等学校化学学报》2024年第5期1-9,共9页Chemical Journal of Chinese Universities

基　　金：国家杰出青年科学基金(批准号:22025403)资助.

摘　　要：单细胞分析能够更加精准地获取生物学信息,避免因平均化分析而丢失单细胞异质性特征,这对于研究阐明细胞代谢和信号通路至关重要.基于纳米电极的电化学分析技术因其高选择性、高灵敏度和高时空分辨率的优点而被广泛用于单细胞分析.本文综合评述了利用纳米电极对单细胞内部生物分子进行定量分析的最新研究进展,介绍了其在生物学研究中的应用,并对该领域面临的问题和未来发展进行了总结与展望.Single-cell analysis enables more accurate acquisition of biological information and avoids the loss of single-cell heterogeneity features from averaging analysis,which is essential for studies to elucidate cell metabolism and signaling pathways.Electrochemical analysis techniques based on nanoelectrodes are widely used in single-cell analysis because of their high selectivity,high sensitivity and high spatiotemporal resolution.In this review,the latest research progress of the quantitative analysis of intracellular biomolecules in single cells using nanoelectrodes is reviewed.Moreover,its application in biological research and the challenges are introduced.

关 键 词：单细胞 单细胞器 生物分子 电化学分析 纳米电极 

分 类 号：O657[理学—分析化学]