糖尿病对大鼠性功能及阴茎海绵体组织形态学、α-SMA表达的影响  

Effects of diabetes mellitus on sexual function,histomorphology andα-smooth muscle actin expression in cavernous body of penis in rats

作　　者：于旭东 张耀圣[2] 商建伟[2] 闫少帅 王旭昀 YU Xudong;ZHANG Yaosheng;SHANG Jianwei;YAN Shaoshuai;WANG Xuyun(The First Clinical Medical College,Beijing University of Chinese Medicine,Beijing 100029,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University,Beijing 100010,China)

机构地区：[1]北京中医药大学第一临床医学院,北京100029 [2]北京中医药大学东直门医院,北京100700 [3]首都医科大学附属北京中医医院,北京100010

出　　处：《长春中医药大学学报》2024年第5期513-516,共4页Journal of Changchun University of Chinese Medicine

基　　金：首都中医药研究专项(17ZY11)。

摘　　要：目的观察糖尿病模型大鼠的性功能情况,探索高血糖继发性功能减退的可能机制。方法实验大鼠随机分为空白组(5只)和糖尿病组(15只),糖尿病动物模型构建成功后,评估2组的性功能;观察2组睾丸组织学特征;测定2组阴茎组织α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的表达情况。结果血清性激素水平显著降低(P<0.01);组织形态学观察显示糖尿病组睾丸组织间质紊乱,阴茎组织平滑肌细胞较空白组减少,而纤维化程度加重;糖尿病组α-SMA的阳性细胞表达显著降低(P<0.01)。结论糖尿病大鼠性激素水平降低、阴茎海绵体平滑肌细胞数减少、阴茎海绵体α-SMA表达量下降、睾丸间质结构紊乱,平滑肌细胞形态的转变以及纤维化程度的加重是本病的重要表型特征,探索针对该表型特征的诊疗将具有重要意义。Objective To observe the sexual function of diabetes model rats and explore the possible mechanism of secondary sexual dysfunction caused by hyperglycemia.Methods The experimental rats were randomly divided into the blank group(n=5)and the diabetes group(n=15).After the diabetes animal model was successfully constructed,the sexual function of two groups was evaluated.The histological characteristics of testis in two groups were observed.The expression ofα-smooth muscle actin(α-SMA)in penile tissue of two groups was measured.Results The levels of serum sex hormones were significantly decreased(P<0.01).Histomorphological observation showed that in the diabetes group the interstitial tissue of testis was disordered;Compared with the blank group,the number of smooth muscle cells in the penis tissue was reduced,and the degree of fibrosis was aggravated.The expression ofα-SMA positive cells in the diabetes group was significantly decreased(P<0.01).Conclusion The decreased levels of sex hormones,the decreased number of smooth muscle cells in cavernous body of penis,the decreased expression ofα-SMA in cavernous body of penis,the disordered structure of interstitial tissue of testis,the morphological changes of smooth muscle cells and the aggravation of fibrosis in the diabetes rats are important phenotypic features of this disease.Exploring the diagnosis and treatment of this disease with this phenotypic features will be of great significance.

关 键 词：糖尿病 勃起功能障碍 性功能 性激素 Α-SMA表达 

分 类 号：R587.2[医药卫生—内分泌]