电针通过恢复肠道微生物群和抑制LPS-TLR4-NF-κB 轴减轻大鼠实验性类风湿性关节炎  

Effect of electroacupuncture on alleviating experimental rheumatoid arthritis in ratsby restoring gut microbiota and inhibiting LPS-TLR4-NF-κB axis

作　　者：周伟[1] 汪昌参 张辽[1] 刘磊 贺飞[1] 杨影 汪富 ZHOU Wei;WANG Changshen;ZHANG Liao;LIU Lei;HE Fei;YANG Ying;WANG Fu(Wanbei Coal and Power Group General Hospital,Suzhou 230041,China)

机构地区：[1]安徽皖北煤电集团总医院,安徽宿州230041

出　　处：《长春中医药大学学报》2024年第5期517-522,共6页Journal of Changchun University of Chinese Medicine

基　　金：安徽省自然科学基金项目(2018085QH363);安徽省宿州市科技计划项目(SZZCZi202210)。

摘　　要：目的探讨电针通过恢复肠道微生物群和抑制LPS-TLR4-NF-κB轴减轻大鼠实验性类风湿性关节炎(RA)的效果。方法从50只清洁级Wistar雄性大鼠中随机抽取10只作为正常组,其余40只建立RA大鼠模型。将成模大鼠随机分为模型组、电针组,其中电针组给予电针疗法治疗,正常组、模型组不作处理,记录不同时间点模型组、电针组关节肿胀度(joint swelling degree,JSD)、关节炎指数(arthritis index,AI)。实验结束后,观察各组关节超微结构变化、肠道微生物群变化、炎症因子水平及LPS-TLR4-NF-κB信号通路因子mRNA、蛋白表达情况。结果干预后,电针组JSD、AI明显低于模型组(P<0.05);与正常组比较,模型组滑膜组织增生,细胞膜破坏严重且不完整,伴大量炎性细胞浸润,电针治疗后,大鼠滑膜组织增生情况、炎性细胞浸润情况、粗面内质网扩张情况明显改善,核膜边缘清晰;与模型组比较,电针组的肠道微生物群相对丰度、炎症因子水平、LPS-TLR4-NF-κB信号通路相关mRNA水平均有改善(P<0.05)。结论电针疗法可有效改善RA大鼠关节症状、抑制炎症因子表达,其机制可能与调节肠道微生物群和抑制LPS-TLR4-NF-κB信号通路有关。Objective To explore the effect of electroacupuncture on alleviating experimental rheumatoid arthritis(RA)in rats by restoring intestinal microbiota and inhibiting LPS-TLR4-NF-κB axis.Methods 10 rats were randomly selected as the normal group from 50 clean Wistar male rats,and the remaining 40 rats were used to establish RA rat models.The model mice were randomly divided into the model group and the electroacupuncture group,in which the electroacupuncture group was treated with electroacupuncture therapy,while the normal group and the model group were not treated.The joint swelling degree(JSD)and arthritis index(AI)in the model group and electroacupuncture group at different time points were recorded.After the experiment,the changes of joint ultrastructure and intestinal microflora,levels of inflammatory factors,and expression of LPS-TLR4-NF-κB signaling pathway factors mRNA and protein in each group were observed.Results After intervention,JSD and AI in the electroacupuncture group were significantly lower than those in the model group(P<0.05).Compared with the normal group,the model group had synovial tissue proliferation,severe and incomplete cell membrane destruction,and a large number of inflammatory cell infiltration;After electroacupuncture treatment,the synovial tissue proliferation,inflammatory cell infiltration,rough endoplasmic reticulum expansion in rats were significantly improved,and the nuclear membrane margin was clear.Compared with the model group,the relative abundance of intestinal microbiota,levels of inflammatory factors,and LPSTLR4-NF-κB signaling pathway related mRNA levels in the electroacupuncture group were improved(P<0.05).Conclusion Electroacupuncture therapy can effectively improve joint symptoms and inhibit the expression of inflammatory factors in RA rats,and its mechanism may be related to the regulation of intestinal microbiota and the inhibition of LPS-TLR4-NF-κB signaling pathway.

关 键 词：类风湿性关节炎 动物实验 电针 肠道微生物群 LPS-TLR4-NF-κB轴 

分 类 号：R593.22[医药卫生—内科学]