小棚养殖凡纳滨对虾“滴星病”的研究  

Study on“Dixing disease”of Litopenaeus vannamei cultured in small shed

作　　者：于志君 王李宝[2] 黎慧[2] 史文军[2] 赵然 万夕和[2] YU Zhijun;WANG Libao;LI Hui;SHI Wenjun;ZHAO Ran;WAN Xihe(National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Jiangsu Institute of Oceanology and Marine Fisheries,Nantong 226007,China)

机构地区：[1]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [2]江苏省海洋水产研究所,南通226007

出　　处：《上海农业学报》2024年第2期94-101,共8页Acta Agriculturae Shanghai

基　　金：江苏省农业科技自主创新资金项目[CX(21)3160];江苏现代农业产业技术体系[JATS(2022)419、JATS(2022)164];江苏省农业重大新品种创制项目(PZCZ201747)。

摘　　要：为探究凡纳滨对虾(Litopenaeus vannamei)“滴星病”的发病原因,从濒死的患病对虾肝胰腺中分离纯化得到一株优势菌MRY0520,并对菌株MRY0520进行了形态学观察和生理生化鉴定及分子生物学研究。结果表明:该菌株为美人鱼发光杆菌美人鱼亚种(Photobacterium damselae subsp.damselae),人工回感健康的凡纳滨对虾可致其出现与自然发病虾相同的症状。该菌对凡纳滨对虾72 h的半数致死量LD50为2.15×10^(5)CFU∕g,药敏试验结果显示MRY0520对新霉素(30μg∕片)、多西环素(30μg∕片)等多种药物敏感。该菌对凡纳滨对虾具有致病性,可致对虾肝胰腺出现明显的病理变化,甚至死亡。To explore the pathogenesis of Litopenaeus vannamei,a dominant strain MRY0520 was isolated from the hepatopancreas of dying diseased shrimp.Meanwhile,the strain MRY0520 was observed morphologically,identified physiologically and biochemically,and analyzed by molecular biology.The results showed that the strain MRY0520 was identified as Photobacterium damselae subsp.damselae,and the artificially infected L.vannamei could cause healthy shrimp exhibit the same symptoms as natural diseased shrimp.The results of artificial challenge showed that the LD50 of the bacteria to L.vannamei in 72 h was 2.15×10^(5)CFU∕g,the drug sensitivity test showed that strain MRY0520 was sensitive to neomycin(30μg∕disc),doxycycline(30μg∕disc)and other drugs.The bacteria was pathogenic to L.vannamei,and could cause obvious pathological changes in the hepatopancreas of the shrimp,even leading to death.

关 键 词：凡纳滨对虾 美人鱼发光杆菌美人鱼亚种 分离鉴定 药敏试验 

分 类 号：S917.1[农业科学—水产科学]