适配体筛选中单链DNA获取方法研究进展  

Research progress on single stranded DNA acquisition methods in adapter screening

作　　者：白亚龙 廖小艳 普春敏 邵毅[1] BAI Yalong;LIAO Xiaoyan;PU Chunmin;SHAO Yi(Institute for Agri-food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;School of Public Health,University of South China,Hengyang 421001,China;Guangzhou Huali Science and Technology Vocational College,Guangzhou 511325,China)

机构地区：[1]上海市农业科学院农产品质量标准与检测技术研究所,上海201403 [2]南华大学公共卫生学院,衡阳421001 [3]广州华立科技职业学院,广州511325

出　　处：《上海农业学报》2024年第2期126-138,共13页Acta Agriculturae Shanghai

基　　金：上海市“科技创新行动计划”农业领域项目(21N31901100);上海市科技兴农项目(2022-02-08-00-12-F01089);上海市农业科学院2022年度农业科技创新支撑领域专项-应用基础研究项目[农科应基2022(09)]。

摘　　要：适配体技术近20年来快速发展,在食品安全、环境监测、法医鉴定、生物医药等领域都得到了高度重视。目前,已有大量研究针对小分子、蛋白质、病毒、细菌、细胞等进行了单链脱氧核糖核酸(ssDNA)适配体筛选。然而ssDNA适配体筛选仍然是一项费时费力的工作,一般都需要经过10轮左右甚至更多轮的ssDNAdsDNA(双链DNA)-ssDNA重复步骤,直到亲和力强的序列成为主要序列,经过测序后,从中挑选一条或几条亲和力最强的序列。显然,在此过程中ssDNA的获取是适配体筛选的关键步骤,也是限速步骤。本文对适配体筛选研究中ssDNA获取的方法进行了系统综述,期望针对适配体筛选中ssDNA次级文库的获取环节可以展开更多更深入的研究,以提高筛选效率。Adaptor technology has developed rapidly in the past 20 years and has received high attention in fields such as food safety,environmental monitoring,forensic identification,and biomedicine.At present,a large number of studies have screened single stranded deoxyribonucleic acid(ssDNA)aptamers for small molecules,proteins,viruses,bacteria,cells,etc.However,ssDNA adapter screening is still a time-consuming and laborious task,usually requiring about 10 or more rounds of ssDNA-dsDNA(double stranded DNA)-ssDNA repeat steps until the sequence with strong affinity becomes the main sequence.After sequencing,one or several sequences with the strongest affinity are selected from them.Obviously,obtaining ssDNA during this process is a crucial step in adapter screening and also a rate limiting step.This article provides a systematic review of methods for obtaining ssDNA in adapter screening research.It is hoped that more in-depth research can be conducted on the acquisition of ssDNA secondary libraries in adapter screening to improve screening efficiency.

关 键 词：适配体 筛选 单链DNA 双链DNA 聚合酶链式反应 

分 类 号：Q78[生物学—分子生物学]