激素浓度组合对成熟油梨枝条伸长和生根的影响  

Effects of Hormone Concentration Combinations on Branch Elongation and Rooting in Mature Avocado

作　　者：成镜 汤倩[3] 黄天带 顾晓川[2] 徐正伟[2] 彭素娜[2] CHENG Jing;TANG Qian;HUANG Tiandai;GU Xiaochuan;XU Zhengwei;PENG Suna(Hainan Natural Rubber and Tropical Forest Research Institute,Haikou,Hainan 571101,China;Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,P.R.China/Cultivation Base of State Key Laboratory Jointly Built by the Province and Ministry/State Key Laboratory Incubation Base for Cultivation&Physiology of Tropical Crops/Engineering Center for Rapid Propagation of Tropical Woody Plants/Haikou Key Laboratory of Innovation of Seedlings of Tropical Plants,Haikou,Hainan 571101,China;Forestry College,Hainan University,Danzhou,Hainan 571737,China)

机构地区：[1]海南天然橡胶与热带林木研究院,海南海口571101 [2]中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地/海南省热带作物栽培生理学重点实验室/热带林木种子种苗工程中心/海口市热带植物种苗创新重点实验室,海南海口571101 [3]海南大学林学院,海南儋州571737

出　　处：《热带农业科学》2024年第3期15-22,共8页Chinese Journal of Tropical Agriculture

基　　金：海南省自然科学基金项目(No.321QN349);海南省优秀团队"橡胶遗传育种"专项资金(No.20210203)。

摘　　要：为了建立抗根腐病成熟油梨砧木的快繁技术体系,通过设置不同浓度(0.1、0.5、1.0、1.5、2.0、2.5、3.0 mg/L)的细胞分裂素(meta-topolin,MT)与不同浓度(0.1、0.5、1.0、1.5 mg/L)的赤霉素(gibberellin,GA3)的组合试验,研究适宜于成熟油梨外植体伸长的培养条件;同时设置不同浓度(0.5、1.0、1.5、2.0、2.5、3.0 mg/L)的吲哚-3-丁酸(indole-3-butyric acid,IBA)的生根培养基,探究最适油梨外植体生根的培养条件。研究发现,伸长试验30 d时(D30)油梨外植体株高增量和茎粗增量的效应量依次是MT浓度×GA_(3)浓度>MT浓度>GA_(3)浓度;对D30叶片增量的效应量依次是MT浓度>MT浓度×GA_(3)浓度>GA_(3)浓度。结果表明,在该研究的测试范围内,最适于伸长的激素浓度组合是MT 2 mg/L、GA_(3)(0.5~1.0)mg/L,而在测试的IBA浓度范围内均没有观察到根系的生长。通过显微结构观察发现,MT浓度为1 mg/L处理的油梨外植体茎木质部(木质部厚度为171.6μm)生长优于MT浓度为0.1 mg/L的处理(木质部厚度为95.3μm),这对后期的成功驯化有一定积极作用。本研究结果可为成熟油梨快繁体系建立提供借鉴。This experiment was conducted to investigate the suitable culture conditions for the elongation of mature oil pear explants by combining cytokinin(meta-tooling,MT)at different concentrations(0.1,0.5,1.0,1.0,and 1.5 mg·L^(-1))and gibberellin(GA_(3))at various concentrations(0.1,0.5,1.0,and 1.5 mg·L^(-1))to study the culture conditions suitable for the elongation of mature avocado explants to establish a fast breeding technology system for root rot-resistant mature oil pear rootstocks,and at the same time,rooting media with different concentrations(0.5,1.0,1.5,2.0,2.5,and 3.0 mg·L^(-1))of indole-3-butyric acid(IBA)were used to determine the optimal culture conditions for the rooting of avocado explants.We investigated the most suitable culture conditions for the rooting of avocado explants.The effects of the elongation test for 30 d(D30)on the increase in plant height and stem thickness of avocado explants were in the order of MT concentration×GA_(3)concentration>MT concentration>GA_(3)concentration,and the effects on the increase in leaf blades of D30 were in the order of MT concentration>MT concentration×GA_(3)concentration>GA_(3)concentration.The most suitable hormone concentration combinations for elongation in the tested range of this study were 2 mg·L^(-1)MT and 0.5-1.0 mg·L^(-1)GA_(3),and no root growth was observed in any of the tested IBA concentration ranges.Microstructural observation revealed that the stem xylem of oil pear explants treated with an MT concentration of 1 mg·L^(-1)(with a xylem thickness of 171.6μm)grew better than that of plants treated with an MT concentration of 0.1 mg·L^(-1)(with a xylem thickness of 95.3μm),indicating that MT had a positive effect on successful domesti-cation at a later stage.The results of this study can be helpful for future fast propagation systems for mature avocado estab-lishment.

关 键 词：成熟油梨 激素 抗根腐病砧木 伸长 生根 驯化 

分 类 号：S532[农业科学—作物学]