紫苏-乳香-没药挥发油对小鼠创口愈合的促进作用  

Promoting Effects of Perilla-Olibanum-Myrrh Essential Oil on Wound Healing in Mice

作　　者：孟庆霖 董红畅 赵冰倩 郝家乐 林珈羽 赵南晰[1] MENG Qinglin;DONG Hongchang;ZHAO Bingqian;HAO Jiale;LIN Jiayu;ZHAO Nanxi(School of Pharmacy,Beihua University,Jilin 132013,China;Jilin Central Hospital,Jilin 132011,China)

机构地区：[1]北华大学药学院,吉林吉林132013 [2]吉林市中心医院,吉林吉林132011

出　　处：《北华大学学报（自然科学版）》2024年第3期354-358,共5页Journal of Beihua University（Natural Science）

基　　金：国家自然科学基金青年基金项目(82104407)。

摘　　要：目的探讨紫苏挥发油与乳香-没药挥发油配伍对小鼠创口愈合的促进作用。方法将ICR小鼠随机分为空白组(CON)、模型组(MOD)、表皮生长因子凝胶组(GEL)、乳香-没药挥发油组(OM,50μL/mL)、紫苏-乳香-没药挥发油组(POM,70μL/mL,V(紫苏挥发油)V(乳香-没药挥发油)=25),除CON组外,其他各组在小鼠背部造一个直径0.8 cm的开放圆形创口。除MOD组外,每只小鼠涂药1次/d,连续14 d,观察小鼠创口愈合情况、病理组织形态,比较3、7、14 d小鼠血清中TNF-α、IL-1β和IL-6的表达情况。结果POM组小鼠创口愈合率大于OM组,且显著高于MOD组(P<0.05);组织病理学检查显示,POM组创口恢复情况优于MOD组;创后3 d,POM组小鼠血清中TNF-α和IL-6水平显著低于MOD组(P<0.05)。结论紫苏-乳香-没药挥发油能减少创口前期炎症反应,促进小鼠创口愈合。Objective To investigate the promoting effects of Perilla essential oil combined with Olibanum-Myrrh essential oil on wound healing in mice.Method ICR mice were randomly divided into control group(CON),model group(MOD),epidermal growth factor gel group(GEL),Olibanum-Myrrh essential oil group(OM,50μL/mL),Perillum-Olibanum-Myrrh essential oil group(POM,70μL/mL,V(Perilla oil)V(Olibanum-Myrrh oil)=25).An open round wound with a diameter of 0.8 cm was made on the back of each mouse except CON group.Except MOD group,each mouse was treated with medicine once a day for 14 days.The wound healing conditions and pathological tissue morphology were observed.The expression of TNF-α,IL-1βand IL-6 in serum of mice were compared on day 3,7 and 14.Results The wound healing rate of POM group was greater than that of OM group,and significantly higher than that of MOD group(P<0.05).Pathological observation showed that the wound recovery of POM group was better than that of MOD group.On day 3 after injury,the serum levels of TNF-αand IL-6 of mice in POM group were significantly lower than those in MOD group(P<0.05).Conclusion Perilla oil combined with Olibanum-Myrrh essential oil can reduce inflammatory response in the early stage of wound and promote wound healing in mice.

关 键 词：紫苏挥发油 乳香-没药 创口愈合 

分 类 号：R961[医药卫生—药理学]