牛奶中四环素类药物残留检测技术研究进展  

Research progress on detection of tetracycline residues in milk

作　　者：高家政 尹营 霍思宇 耿旭浩 孙玲玲 陈冬东[1] 薛占永 彭涛[1] GAO Jiazheng;YIN Ying;HUO Siyu;GENG Xuhao;SUN Lingling;CHEN Dongdong;XUE Zhanyong;PENG Tao(Chinese Academy of Inspection and Quarantine,Beijing 100176,China;School of Life Science and Food Engineering,Hebei University of Engineering,Handan 056038,China;Shandong Shuiyue Inspection and Testing Co.,Ltd.,Tai'an 271000,China;Cas(Beijing)Science and Technology Co.,Ltd.,Beijing 100176,China)

机构地区：[1]中国检验检疫科学研究院,北京100176 [2]河北工程大学生命科学与食品工程学院,河北邯郸056038 [3]山东水岳检验检测有限公司,山东泰安271000 [4]检科院(北京)科学技术有限公司,北京100176

出　　处：《黑龙江畜牧兽医》2024年第8期19-25,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家重点研发计划项目“现场执法快检产品多维评价技术研究与认证应用”(2022YFF1101004)。

摘　　要：四环素类药物(tetracyclines,TCs)是一种广谱抗生素,常用于治疗奶牛疾病,但使用不当易导致药物残留,影响牛奶品质与食用安全。目前,快捷、高效、一次可检测多种药物是兽药残留分析技术的发展方向。笔者综述了牛奶TCs残留分析技术中常见的样品前处理、纯化和药物测定方法,包括液-液萃取(liquid-liquid extraction,LLE)和液-液微萃取(liquid-liquid micro extraction,LLME)等前处理方法,液-液分配(liquid-liquid partition,LLP)、固相萃取(solid-phase extraction,SPE)、QuEChERS等纯化方法,以及薄层色谱(thin-layer chromatography,TLC)法、化学发光(chemiluminescence,CL)法、毛细管电泳(capillary electrophoresis,CE)法、高效液相色谱(high performance liquid chromatography,HPLC)法、液相色谱串联质谱(liquid chromatography tandem mass spectrometry,LC-MS/MS)法、免疫分析法、分光光度法和电化学分析法等药物测定方法,分析了各种方法的优缺点,旨在为牛奶中TCs残留检测提供参考。Tetracycline(TCs)is a broad-spectrum antibiotic,which is often used in the treatment of dairy cow diseases.Improper use of tetracyclines can easily lead to drug residues and affect the quality and safety of milk.At present,the development direction of veterinary drug residue analysis technology is fast and efficient detection of multiple drugs at one time.The common sample pretreatment,purification and drug determination methods in milk TCs residue analysis were reviewed,including liquid-liquid extraction(LLE)and liquid-liquid micro extraction(LLME),liquid-liquid partition(LLP),solid-phase extraction(SPE),QuEChERS and other purification methods,thin-layer chromatography(TLC),chemiluminescence(CL),capillary electrophoresis(CE),high performance liquid chromatography(HPLC),liquid chromatography tandem mass spectrometry(LC-MS/MS),immunoassay,spectrophotometry and electrochemical assay and other methods for drug determination.The advantages and disadvantages of various methods were analyzed in order to provide reference for the detection of TCs residues in milk.

关 键 词：牛奶 四环素类药物 残留 样品前处理方法 纯化方法 药物测定方法 液液萃取 固相萃取 高效液相色谱 

分 类 号：S859.84[农业科学—临床兽医学]