低pH值养殖水对罗非鱼肠道菌群结构的影响  

Effects of low pH value of culture water on intestinal microflora structure of Tilapia

作　　者：龚钰雯 黄春红 覃日锐 周玥琪 何欢 赵早亚 易弋 CONG Yuwen;HUANG Chunhong;QIN Rirui;ZHOU Yueqi;HE Huan;ZHAO Zaoya;YI Yi(College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Guangxi Key Laboratory of Green Processing of Sugar Resources,Liuzhou 545006,China;Liujiang District Agriculture and Rural Comprehensive Development Service Center,Liuzhou 545006,Ckina;Liujiang Distiel Agrieulural Science and Technology Extension Center,Liuzhou 545006,China)

机构地区：[1]广西科技大学生物与化学工程学院广西糖资源绿色加工重点实验室,广西柳州545006 [2]柳江区农业农村综合发展服务中心,广西柳州545006 [3]柳江区农业科技推广中心,广西柳州545006

出　　处：《黑龙江畜牧兽医》2024年第8期117-125,共9页Heilongjiang Animal Science And veterinary Medicine

基　　金：柳州市科技计划项目(2020NACB0802);柳州市柳江区科学研究与技术开发计划项目。

摘　　要：为了解养殖水低pH值对罗非鱼肠道菌群结构的影响,试验将36尾驯化后的吉富罗非鱼随机分为两组(空白组和处理组),每组3个重复,每个重复6尾,空白组养殖水pH值≈7.0,处理组养殖水pH值≈4.0,试验期为14 d,试验结束后提取两组肠道微生物基因组DNA,扩增16S rDNA基因,利用Illumina Miseq PE300平台进行测序后,对两组的肠道菌群的Alpha多样性指数、物种组成及优势菌群和差异菌群进行分析,并进行PICRUSt2功能预测和BugBase表型预测。结果表明:处理组肠道微生物的香农指数显著高于空白组(P<0.05),辛普森指数显著低于空白组(P<0.05),群落丰富度指数两组间差异不显著(P>0.05)。空白组和处理组肠道中菌门数量分别为22个和28个,共有的菌门数为18个;空白组和处理组肠道中菌属数量分别为235个和373个,共有的菌属数为137个;空白组和处理组肠道中扩增子序列变体(amplicon sequence variants, ASVs)数量分别为458个和791个,共有的ASVs数量为162个。在门水平上,处理组和空白组的优势菌群均为放线菌门和变形菌门;在属水平上,处理组中的优势菌群为代尔夫特菌属、分枝杆菌属、红球菌属和微小金杆菌属等,空白组的优势菌群为代尔夫特菌属、红球菌属、微小金杆菌属等。在门水平上,处理组肠道中浮霉菌门相对丰度显著低于空白组(P<0.05);在属水平上,处理组肠道中分枝杆菌属、小粒凯斯特亚菌属、金色类节杆菌属、双头菌属、短小杆菌属、甲基杆菌属-甲基红色杆菌属、村氏菌属和黄色杆菌属相对丰度极显著高于空白组(P<0.01),α-变形菌属相对丰度极显著低于空白组(P<0.01),赖氏菌属和博斯氏菌属相对丰度显著高于空白组(P<0.05),代尔夫特菌属、红球菌属、微小金杆菌属、桫椤菌属相对丰度显著低于空白组(P<0.05)。在PICRUSt2功能预测中,差异ASVs主要涉及细胞代谢、环境信息处理和遗传信息处理等功In order to understand the effects of low pH value of culture water on intestinal microflora structure of Tilapia,36 domesticated Tilapia were randomly divided into two groups(blank group and treatment group)with 3 replicates in each group and 6 fish in each replicate.The pH value of culture water in the blank group was≈7.0,and that of culture water in the treatment group was 4.0.The experiment lasted for 14 days.After the experiment,the intestinal microbial genomic DNA of the two groups was extracted;16S rDNA gene was amplified,and after sequencing by Illumina Miseq PE300 platform,the Alpha diversity index,species composition,dominant bacteria and different bacteria of the intestinal flora of the two groups were analyzed.PICRUSt2 function prediction and BugBase phenotype prediction were also performed.The results showed that the Shannon index of intestinal microorganisms in treatment group was significantly higher than that in blank group(P<0.05);the Simpson index of intestinal microorganisms in treatment group was signficantly lower than that in blank group(P<0.05),and there was no significant difference in community richness index between the two groups(P>0.05).The number of microphyla in the intestine of blank group and treatment group was 22 and 28,respectively,and the mutual number of microphyla was 18.The number of bacteria genera in the intestine of blank group and treatment group was 235 and 373,respectively,and the number of mutual bacteria genera was 137.The number of ASVs in the intestine of blank group and treatment group was 458 and 791,respectively,and the mutual number of ASVs was 162.At gate level,Actinomyces and Proteobacteria were dominant bacteria in both treatment group and blank group.At the genus level,the dominant bacteria in the treatment group were Delfteria,Mycobacterium,Rhodococcus and Metallobacillus parvus,etc.,while the dominant bacteria in the blank group were Delfteria,Rhodococcus and Metallobacillus parvus.At the gate level,the relative abundance of pontospora in intestinal tra

关 键 词：罗非鱼 PH值 肠道菌群 物种多样性 功能预测 

分 类 号：S965.125[农业科学—水产养殖]