11份益母草种质材料苗期耐盐性评价与鉴定  

Evaluation and identification of salt tolerance of 11 Leonurus germplasm lines at the seedling stage

作　　者：孟晨 鲁雪莉 宋亦汝 张成省 李义强[1,2,3] 项海芹 徐宗昌 MENG Chen;LU Xue-li;SONG Yi-ru;ZHANG Cheng-sheng;LI Yi-qiang;XIANG Hai-qin;XU Zong-chang(Marine Agriculture Research Center,Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266100,China;National Center of Technology Innovation for Comprehensive Utilization of Saline-alkali Land,Dongying 257345,China;Qingdao Key Laboratory of Coastal Saline-alkali Land Resources Mining and Biological Breeding,Qingdao 266100,China;College of Agriculture,Qingdao Agricultural University,Qingdao 266109,China;Dongying Xinshengdai Biotechnology Co.,Ltd.,Dongying 257300,China)

机构地区：[1]中国农业科学院烟草研究所海洋农业研究中心,山东青岛266100 [2]国家盐碱地综合利用技术创新中心,山东东营257345 [3]青岛市滨海盐碱地资源挖掘与生物育种重点实验室,山东青岛266100 [4]青岛农业大学农学院,山东青岛266109 [5]东营新生带生物科技有限公司,山东东营257300

出　　处：《草业学报》2024年第5期196-203,共8页Acta Prataculturae Sinica

基　　金：中国农业科学院科技创新工程(CAAS-ZDRW202201,ASTIP-TRIC06);青岛市科技惠民示范引导专项(23-3-3-1-zyyd-nsh)资助。

摘　　要：土壤盐渍化是制约我国农业发展的重要问题之一。利用药用植物对盐碱地进行生物改良,是盐碱地资源化利用的有效手段。本研究用2%浓度的NaCl对11份益母草苗期材料进行盐胁迫处理,测定株高、根长、叶面积、地上鲜重、根鲜重等12个指标,采用主成分分析、隶属函数分析及耐盐性综合评价分析对各样本的耐盐性进行了综合评价。结果表明,益母草各指标差异较大,变异系数为13.58%~59.89%,地上鲜重、地上干重、根鲜重及叶面积4个指标受盐胁迫的影响较大,且12个性状指标间存在不同程度的相关性。叶绿素含量、主根直径、地上部分及根的干鲜重可以作为益母草苗期耐盐性评价指标。综合耐盐等级划分和耐盐性综合评价结果,筛选到苗期较为耐盐的益母草材料HY199和HY203。以上结果将会为益母草的盐碱地种植提供理论依据。Soil salinization is one of the main factors restricting agricultural development in China.The use of medicinal plants for biological improvement of saline alkali land is an effective means of resource utilization of it.In this study,11 Leonurus heterophyllus materials were treated with 2%w/v NaCl at the seedling stage to impose salt stress,and 12 indexes including plant height,root length,leaf area,above-ground fresh weight,and root fresh weight were measured.Multivariate statistical analysis methods such as principal component analysis and membership function analysis were used to evaluate the salt tolerance of each sample.The results showed that there were significant differences in various indexes of L.heterophyllus,with the coefficient of variation ranging from 13.58%to 59.89%.The four indexes of the above-ground fresh weight,above-ground dry weight,root fresh weight,and leaf area were greatly affected by salt stress,and there were varying degrees of correlation among the 12 indexes.Chlorophyll content,main root diameter,dry and fresh weight of above-ground parts as well as dry and fresh weight of roots were identified as indicators for evaluating the salt tolerance of L.heterophyllus at the seedling stage.Based on the salt tolerance score and the comprehensive evaluation of salt tolerance,HY199 and HY203 were identified as the most salt-tolerant L.heterophyllus.The above results provide theoretical guidelines for the cultivation of L.heterophyllus in saline-alkali areas.

关 键 词：益母草 耐盐性 隶属函数分析 主成分分析 

分 类 号：S567.2[农业科学—中草药栽培]