UHPLC-MS/MS测定化妆品中67种植物提取物标识成分  

Determination of 67 Indicative Components from Plant Extracts in Cosmetics by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：张秋炎 黄芳 梁维维 廖均涛 吴惠勤 罗辉泰 ZHANG Qiu-yan;HUANG Fang;LIANG Wei-wei;LIAO Jun-tao;WU Hui-qin;LUO Hui-tai(Guangdong Provincial Engineering Research Center for Quality and Safety of Traditional Chinese Medicine,Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology,Institute of Analysis,Guangdong Academy of Sciences(China National Analytical Center,Guangzhou),Guangzhou 510070,China)

机构地区：[1]广东省科学院测试分析研究所(中国广州分析测试中心),广东省化学测量与应急检测技术重点实验室,广东省中药质量安全工程技术研究中心,广东广州510070

出　　处：《分析测试学报》2024年第5期663-673,共11页Journal of Instrumental Analysis

基　　金：广东省科学院打造综合产业技术创新中心行动资金项目“高端医疗健康与高效生物转化关键技术研究及应用”(2022GDASZH-2022010110)。

摘　　要：建立了超高效液相色谱-串联质谱(UHPLC-MS/MS)测定化妆品中67种植物提取物标识成分的方法。化妆品样品经甲醇(含1%甲酸)超声提取,样液在0.1%甲酸溶液-乙腈流动相体系下经Agilent RRHD Eclipse Plus Zorbax C18(3.0 mm×100 mm,1.8μm)色谱柱梯度洗脱分离。采用电喷雾正、负离子模式分别对67种成分进行定性定量分析,其中正离子采用多反应监测(MRM)方式,负离子采用动态多反应监测(DMRM)方式,以基质匹配外标法定量。以膏霜、乳液基质为代表,67种成分在各自质量浓度范围内线性关系良好,相关系数(r^(2))均大于0.99;检出限为0.0026~1.7μg/g,定量下限为0.0080~5.0μg/g;3个不同加标水平下,膏霜、乳液基质的平均回收率分别为85.6%~117%和82.7%~116%,相对标准偏差(RSD,n=6)分别为1.0%~12%和0.90%~12%。该法简单快速,灵敏度高,适用于化妆品中67种植物提取物标识成分的定性鉴定和含量测定。A new method for the simultaneous and rapid determination of 67 indicative components from plant extracts in cosmetics by ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)was established.The cosmetic samples were dispersed with methanol(1%formic acid)and ultrasonic extraction.The filtrate was analyzed by a Agilent RRHD Eclipse Plus Zorbax C18 chromatographic column(3.0 mm×100 mm,1.8μm)with mobile phase consisted of 0.1%formic acid aqueous solution and acetonitrile.67 indicative components from plant extracts were analyzed in the electrospray ionization source,including positive ion scanning with multiple reaction monitoring mode(MRM)and negative ion scanning with dynamic multiple reaction monitoring mode(DMRM)respectively.Creams and lotions were choosed as the representative matrix.The 67 indicative components showed good linearity with the correlation coefficients(r^(2))greater than 0.99 in their respective mass concentration ranges.The detection limits(LODs)of 67 indicative components were in the range of 0.0026-1.7μg/g,and the quantitation limits(LOQs)were in the range of 0.0080-5.0μg/g.For cream and lotion samples,the average recoveries of 67 indicative components at three different concentration levels ranged of 85.6%-117%and 82.7%-116%,respectively;with relative standard deviations(RSDs,n=6)ranged of 1.0%-12%and 0.90%-12%.The established method is simple,rapid,effective,high sensitive,and is suitable for the qualitative identification and content determination of 67 indicative components from plant extracts in cosmetics.

关 键 词：超高效液相色谱-串联质谱法 化妆品 植物提取物 标识成分 

分 类 号：O657.7[理学—分析化学] TQ658[理学—化学]