拟南芥组织特异性表达FHY3对fhy3far1细胞死亡的影响  

Effects of Tissue-specific Expression of FHY3 on Cell Death of fhy3far1 in Arabidopsis Thaliana

作　　者：徐云巧 朱凤晨 马琳 秦晓春 XU Yunqiao;ZHU Fengchen;MA Lin;QIN Xiaochun(School of Biological Science and Technology,University of Jinan,Jinan 250022,Shandong,China)

机构地区：[1]济南大学生物科学与技术学院,山东济南250022

出　　处：《济南大学学报（自然科学版）》2024年第3期369-375,共7页Journal of University of Jinan(Science and Technology)

基　　金：国家重点研发计划项目(2018YFD1000501);国家自然科学基金项目(31900211)。

摘　　要：为了探究植物光信号蛋白FHY3及其同源蛋白FAR1在叶片细胞死亡调控过程中的分子机制,在拟南芥fhy3far1双突变体背景下分别在维管束及叶肉细胞中表达FHY3。结果表明:在fhy3far1双突变体维管束中特异性表达FHY3可完全改善fhy3far1的细胞死亡表型,而在fhy3far1双突变体叶肉细胞中特异性表达FHY3仅可部分缓解fhy3far1叶片细胞死亡表型,揭示了FHY3调控细胞死亡过程主要是在维管束中行使功能。To investigate the mechanism of light signaling protein FHY3 and its homologous protein FAR1 on the leaves cell death,two sets of transgenic plants in Arabidopsis thaliana that FHY3 particularly expressed in the vascular bundle or mesophyll cells were generated in the fhy3far1 double mutant background.The results show that FHY3 expressed in vascular bundles completely rescue the leaves cell death phenotype of fhy3far1,however,FHY3 specific expressed in the mesophyll cells only largely rescue the cell death phenotype of fhy3far1,so FHY3 plays an essential role in regulating leaf cell death mainly occurring in the vascular bundles.

关 键 词：光信号蛋白 维管束 叶肉细胞 细胞死亡 拟南芥 

分 类 号：Q78[生物学—分子生物学]