五指毛桃不同部位补骨脂素合成酶基因的表达及其代谢产物含量  

Expression of Psoralen Synthase Gene and Effect of Its Product Synthesis in Different Parts of Ficus hirta Vahl

作　　者：杨琳[1] 杨帆 张杭颖[1] 黄勃诚 邱清华 张君诚[1] YANG Lin;YANG Fan;ZHANG Hangying;HUANG Bocheng;QIU Qinghua;ZHANG Juncheng(Fujian Provincial Medical Plant Exploitation and Utilization Engineering Research Center,Sanming University,Sanming,Fujian 365004,China;School of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350000,China)

机构地区：[1]三明学院药用植物开发利用福建省高校工程研究中心,福建三明365004 [2]福建农林大学食品科学学院,福建福州350000

出　　处：《热带作物学报》2024年第4期674-681,共8页Chinese Journal of Tropical Crops

基　　金：福建省科技厅面上项目(No.2020J01382);福建省教育厅中青年教师教育科研项目(No.JAT220359)。

摘　　要：为探究五指毛桃(Ficus hirta Vahl)不同部位补骨脂素合成酶基因(psoralen synthetase,PS)表达与补骨脂素含量的关系,本研究分别采用实时荧光定量PCR法和高效液相色谱法测定来自广西和福建的五指毛桃根、茎、叶和果实PS基因的表达量及补骨脂素含量,分析不同五指毛桃种质不同部位PS基因表达及其代谢产物含量的差异。结果显示:PS基因在广西和福建五指毛桃的根、茎、叶和果实中均有表达,且其表达量具有组织特异性,在根中的相对表达量最高(侧根高于主根),叶中次之(全缘叶高于缺刻叶),茎和果实的表达量极低。使用高效液相色谱测得广西和福建的五指毛桃中补骨脂素含量存在差异,在2个五指毛桃种质的主根、侧根、缺刻叶、全缘叶、茎、果实中均有积累,侧根中的含量最高,分别为3.206、2.947 mg/g,主根次之(2.136、0.419 mg/g),缺刻叶中的含量分别为0.022、0.028 mg/g,全缘叶中的含量分别为0.069、0.035 mg/g,茎(0.003、0.003 mg/g)和果实(0.002、0.001 mg/g)中含量较低。五指毛桃PS基因表达与补骨脂素含量具有相关性,该研究结果为解析PS基因在五指毛桃补骨脂素合成中的作用提供参考。To explore the relationship between the expression of psoralen synthase gene(PS)and psoralen synthesis,quantitative real-time PCR(qPCR)and high performance liquid chromatography(HPLC)were used to determine the expression of PS and the content of psoralen in roots,stems,leaves and fruits of Ficus hirta Vahl(FH)from Guangxi and Fujian.PS was expressed in roots,stems,leaves and fruits of Guangxi and Fujian FH,and the expression was tissue-specific.The relative expression level in roots was the highest(expression in branch roots was higher than that in principal roots),followed by leaves(expression in entire margined leaves were higher than that in incised leaves),and the expression levels in stems and fruits were extremely low.The psoralen contents of FH had some differences from the two producing areas.They were accumulated in the principal root,branch root,entire margined leaf,incised leaf,stem and fruit from Guangxi and Fujian provinc FH,and the contents were the highest in branch roots,which was 3.206 mg/g and 2.947 mg/g respectively,followed by principal root(2.136 mg/g and 0.419 mg/g).The content in incised leaves was 0.022 mg/g and 0.028 mg/g respectively,that in entire margined leaves was 0.069 mg/g and 0.035 mg/g.The psoralen content of stem(0.003 mg/g and 0.003 mg/g)and fruit(0.002 mg/g and 0.001 mg/g)was lower than other parts.The psoralen content was correlated with the expression of PS.The study would provide insights into understanding the role of PS in the psoralen metabolic in Ficus hirta Vahl.

关 键 词：五指毛桃 补骨脂素 补骨脂素合成酶 基因表达 工艺优化 含量 

分 类 号：S567.19[农业科学—中草药栽培]