辣椒种质资源PMMoV抗性基因检测与抗性鉴定  

Resistance Gene Detection and Resistance Identification of PMMoV in Pepper Germplasm

作　　者：陈建分 曹振木[1] 秦于玲[1] 申龙斌[1] 刘维侠[1] 朱丹 吴怡婷 刘子记[1] 王旭[2] CHEN Jianfen;CAO Zhenmu;QIN Yuling;SHEN Longbin;LIU Weixia;ZHU Dan;WU Yiting;LIU Ziji;WANG Xu(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;School of Horticulture,Hainan University,Haikou,Hainan 570208,China)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南海口571101 [2]海南大学园艺学院,海南海口570208

出　　处：《热带作物学报》2024年第4期682-690,共9页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金面上项目(No.322MS132);中央级公益性科研院所基本科研业务费专项(No.1630032022009)。

摘　　要：辣椒轻斑驳病毒(Pepper mild mottle virus,PMMoV)是世界范围内最严重的病原体之一,目前已报道的PMMoV包括P_(0)、P_(1)、P_(1,2)、P_(1,2,3)、P_(1,2,3,4)五种致病型。辣椒轻斑驳病毒寄主范围广、致病力强,严重影响辣椒的产量和品质,开展抗病育种是防治该病毒最为经济有效的手段。本课题组前期研究已明确了感染海南儋州辣椒种质资源圃的PMMoV为P_(1,2)致病型,为了筛选抗PMMoV的辣椒种质资源,以110份种质为材料,利用已报道的与L^(3)连锁的分子标记对辣椒种质进行检测。结果表明:在110份材料中共检测到46份辣椒种质含有与L^(3)连锁的分子标记。采用苗期人工接种鉴定方法,对含有抗性分子标记的46份辣椒种质接种PMMoV P_(1,2)致病型进行抗性鉴定。接种5 d后调查局部症状,接种20 d后调查系统症状,计算病情指数。结果表明:不同的辣椒种质在抗病性上存在显著差异,各种质的病情指数在8.86~60.00之间,对PMMoV P_(1,2)致病型表现高抗(HR)的种质为CF19-34m和19FB6-1;表现抗病(R)的种质为CCJ93-1;表现中抗(MR)的种质包括17YB32、17SCa28m、15SM39-2、14SM555×14SM1、14SM567-2、14SM526-1、14SM565-1、14SM502-1、14SM519-1、14SM504m、14SM503m、14SM519-3、14SM518-2、14SM519-2、CCJ52-1等34份;表现感病(S)的种质包括14SM514m、CCJ22-1、CCJ113-2、CCJ135-1、CCJ157-1、L537-1m、L545、CCJ87-2、CCJ133-1,获得的高抗、抗病、中抗种质占总鉴定材料的80.43%。本研究结果为创制抗辣椒轻斑驳病毒种质资源和培育抗性品种奠定基础。Pepper mild mottle virus(PMMoV)is one of the most serious pathogens worldwide.Currently,five pathogenic types of PMMoV have been reported,including P_(0),P_(1),P_(1,2),P_(1,2,3) and P_(1,2,3,4).The wide host range and strong pathogenicity of PMMoV seriously affect the yield and quality of pepper,and breeding for disease resistance is the most economical and effective means to control this virus.Our previous study determined that PMMoV infected with Danzhou pepper germplasm nursery in Hainan is the P_(1,2) pathogenic type.In order to screen PMMoV-resistant pepper germplasm resources,110 germplasms were tested for PMMoV resistance using the reported L^(3)-linked molecular markers.46 pepper germplasms containing molecular markers of resistance were inoculated with PMMoV P_(1,2) pathogenic type by inoculation at seeding stage.Local symptoms were investigated 5 d after inoculation,and systemic symptoms were investigated 20 d after inoculation,and disease indices were calculated.The results showed that there were sig-nificant differences in disease resistance among different pepper germplasms,and the disease indexes of various germplasms ranged from 8.86-60.00.The germplasms showing high resistance(HR)to PMMoV P_(1,2) pathogenic type were CF19-34m and 19FB6-1.The germplasms showing resistance(R)wasCCJ93-1.The germplasms showing medium-resistance(MR)included 17YB32,17SCa28m,15SM39-2,14SM555×14SM1,14SM567-2,14SM526-1,14SM565-1,14SM502-1,14SM519-1,14SM504m,14SM503m,14SM519-3,14SM518-2,14SM519-2,CCJ52-1,etc.The presenting susceptible(S)germplasms were 14SM514m,CCJ22-1,CCJ113-2,CCJ135-1,CCJ157-1,L537-1m,L545,CCJ87-2 and CCJ133-1,and the obtained disease-resistant germplasms accounted for 80.43%of the total identified materials.The results of this study would lay the foundation for creating germplasm resources resistant to pepper mild mottle virus and breeding resistant varieties.

关 键 词：辣椒 种质资源 PMMoV 分子标记 抗病性鉴定 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]