89份香蕉种质资源SRAP分子标记亲缘关系分析  

Genetic Relationship Analysis among 89 Banana Germplasms Based on SRAP Molecular Markers

作　　者：周海琪 夏玲 吕顺 曾莉莎 王芳 黄晓彦 陈东仪 刘文清 梁少丽 刘丽琴 ZHOU Haiqi;XIA Ling;LYU Shun;ZENG Lisha;WANG Fang;HUANG Xiaoyan;CHEN Dongyi;LIU Wenqing;LIANG Shaoli;LIU liqin(Dongguan Agricultural Research Centre,Dongguan,Guandong 523000,China)

机构地区：[1]东莞市农业科学研究中心,广东东莞523000

出　　处：《热带作物学报》2024年第4期703-711,共9页Chinese Journal of Tropical Crops

基　　金：东莞市2021年度省乡村振兴战略专项资金“大专项+任务清单”(No.20211800400052);广东省级农业科技创新及推广项目“香蕉菠萝产业技术体系创新团队”(No.2023KJ109);2022年东莞市科技特派员项目(No.20221800500062)。

摘　　要：香蕉分类是一个难题,利用形态学特征进行分类已无法满足需要。分子标记技术逐渐被应用到香蕉品种(系)的种群鉴定与分类、亲缘关系分析和遗传多样性研究等方面。本研究采用SRAP分子标记技术对89份香蕉种质资源的亲缘关系进行分析。利用10对正反引物两两组合成100对引物组合,从中筛选出扩增条带易于识别、带型清晰、多态性高的13对SRAP引物,共扩增出170条可辨认条带,平均每对引物扩增13.08条,其中表现出多态性的条带有140条,多态性比率为79.00%。89份香蕉种质资源的相似系数的变化范围在0.241~1.000之间,遗传多样性非常丰富,来源于同一个地方或者来源不同地方的同一类型的野生蕉的相似性系数较高。当相似性系数为0.49时可以将这89份香蕉资源分成六大类,包括香牙蕉、贡蕉和龙牙蕉为主的第一大类(AAA、AA、AAB基因型),粉大蕉、粉蕉和BB类野蕉为主的第二大类(ABB、BB基因型),大蕉、阿宽蕉类野蕉为主的第三大类,以及红花蕉、地涌金莲、蝎尾蕉分别所属的第四、五、六类群。其结果与现行的经典分类结论基本一致,只有部分香蕉种质分类地位有差异,说明用SRAP标记对香蕉种质资源进行分类是可行的。同时,栽培类香牙蕉、贡蕉和龙牙蕉具有相同的祖先,与AA基因型野蕉亲缘关系近,粉蕉、粉大蕉与BB基因型的野蕉亲缘关系较近,不同基因组的香蕉种质资源聚到一起,说明香蕉种质资源的起源影响后代的亲缘关系,单一的分类方法无法将香蕉种质资源完全区分。此外,大蕉与阿宽蕉亲缘关系较近,但是无法分辨大蕉是否含有A和B基因组,因此大蕉基因型和遗传背景有待进一步研究。Banana classification is a difficult problem,using morphological features to classify is unable to meet the needs.Molecular marker technology has been gradually applied to population identification and classification,genetic relationship analysis and genetic diversity research of banana varieties(lines).In this paper,SRAP molecular marker technology was used to analyze the genetic relationship of 89 banana germplasm resources.Using 10 pairs of positive and negative primers to form 100 pairs of primer combinations,and 13 pairs of SRAP primers with easy identification,clear band type and high polymorphism were screened out.170 identifiable bands were amplified by the 13 pairs of primers,with an average of 13.08 bands per pair of primers,of which 140 bands showed polymorphism,with a polymorphism ratio of 79.00%.The similarity coefficient of 89 banana germplasm resources varied from 0.241 to 1.000,and the genetic diversity was very rich.The similarity coefficient of the same type of wild banana from the same place or different places was high.When the similarity coefficient was 0.49,the 89 banana resources could be divided into six groups.The first major category were AAA group Xiangyajiao,AA group Gongjiao,AAB group Longyajiao and AA group wild banana(AAA,AA,AAB).The second major category were ABB group Fenjiao,ABB group Fendajiao and BB group wild banana(ABB,BB).The third major category were Dajiao and a banana wild relative Musa itinerans.M coccinea,Musella lasiocarpa,Heliconia belonged respectively to the fourth,fifth and sixth category.The results are basically consistent with the current classical classification conclusion.Only some banana germplasm have different classification status,indicating that it is feasible to use SRAP markers to classify banana germplasm resources.At the same time,the cultivated Xiangya banana,Gongya banana and Longya banana have the same ancestry,and are close to the wild banana of AA genotype,while the wild banana of BB genotype is close to the pink banana,the pink banana and the wi

关 键 词：香蕉 SRAP 聚类分析 亲缘关系 

分 类 号：S668.1[农业科学—果树学]