木犀草素改善脊髓损伤大鼠血清诱导的心肌细胞死亡  

Luteolin improves myocardial cell death induced by serum from rats with spinal cord injury

作　　者：张稳稳 徐梦如 田园[3] 张立飞 师姝 王宁[3] 原媛[3] 王丽[3,4] 郝海虎[1,2] Zhang Wenwen;Xu Mengru;Tian Yuan;Zhang Lifei;Shi Shu;Wang Ning;Yuan Yuan;Wang Li;Hao Haihu(First Clinical College,Shanxi University of Chinese Medicine,Jinzhong 030600,Shanxi Province,China;Department of Orthopedics,Bethune Hospital,Shanxi Academy of Medical Sciences,Third Hospital of Shanxi Medical University,Taiyuan 030000,Shanxi Province,China;Basic Medical Research Center,Shanxi Medical University,Jinzhong 030600,Shanxi Province,China;Department of Pathology,School of Basic Medicine,Shanxi Medical University,Jinzhong 030600,Shanxi Province,China)

机构地区：[1]山西中医药大学第一临床学院,山西省晋中市030600 [2]山西医科大学第三医院山西白求恩医院山西医学科学院骨科,山西省太原市030000 [3]山西医科大学,基础医学研究中心,山西省晋中市030600 [4]山西医科大学基础医学院病理教研室,山西省晋中市030600

出　　处：《中国组织工程研究》2025年第1期38-43,共6页Chinese Journal of Tissue Engineering Research

基　　金：山西省基础研究计划——自然科学研究面上项目(202103021224347);项目负责人:郝海虎。

摘　　要：背景:脊髓损伤导致的心功能不全是脊髓损伤患者死亡的重要原因,但具体机制并不清楚。因此,揭示脊髓损伤患者出现心功能不全的机制对于提高患者的生存质量和生存率有重要意义。目的:探讨木犀草素改善脊髓损伤大鼠血清诱导心肌细胞死亡的作用机制。方法:使用Allen’s冲击仪损伤雄性SD大鼠脊椎T9-T11,进行脊髓损伤造模,设置假手术组作为对照,取各组大鼠血清。将H9c2细胞分为空白对照组、假手术大鼠血清组、脊髓损伤大鼠血清组以及木犀草素预处理组,空白对照组为普通培养基培养,假手术大鼠血清组使用含体积分数10%假手术大鼠血清的培养基培养,脊髓损伤大鼠血清组使用含体积分数10%脊髓损伤大鼠血清的培养基培养,木犀草素预处理组预先使用含有终浓度为20μmol/L木犀草素的培养基培养心肌细胞4 h,之后更换含体积分数10%脊髓损伤大鼠血清的培养基培养,培养24 h后使用CCK-8法检测各组H9c2心肌细胞的存活率,采用Western blot检测各组H9c2心肌细胞自噬相关蛋白LC3及p62的蛋白表达。结果与结论:与空白对照组相比,假手术大鼠血清组细胞存活率无明显改变(P>0.05);与假手术大鼠血清组相比,脊髓损伤大鼠血清组细胞存活率显著降低(P<0.01),LC3蛋白表达显著降低(P<0.05),p62蛋白表达显著升高(P<0.05);与脊髓损伤大鼠血清组相比,木犀草素预处理组细胞存活率明显回升(P<0.0001),LC3蛋白表达显著升高(P<0.05),p62蛋白表达显著降低(P<0.05)。结果表明:木犀草素可能通过促进自噬进而改善脊髓损伤大鼠血清诱导的心肌细胞死亡。BACKGROUND:Cardiac dysfunction due to spinal cord injury is an important factor of death in patients with spinal cord injury;however,the specific mechanism is still not clear.Therefore,revealing the mechanism of cardiac dysfunction in spinal cord injury patients is of great significance to improve their quality of life and survival rate.OBJECTIVE:To investigate the mechanism of luteolin in improving serum-induced myocardial cell death in spinal cord injury rats.METHODS:Allen's impact instrument was used to damage the spine T9-T11 of male SD rats to establish a spinal cord injury model meanwhile a sham operation group was set as the control group.The serum of rats of each group was collected.H9c2 cells were divided into a blank control group,a sham operated rat serum group,a spinal cord injury rat serum group and a luteolin pretreatment group.The cells in blank control group were only cultured with ordinary culture medium.The cells in the sham operated rat serum group were treated with medium containing 10%serum from sham operated rat.The cells in the spinal cord injury rat serum group were treated with medium containing 10%serum from spinal cord injury rat.The cells in the luteolin pretreatment group were precultured with a final concentration of 20μmol/L luteolin for 4 hours and then changed to a medium containing 10%rat serum from spinal cord injury rat.After 24 hours of culture,the survival rate of each group of H9c2 cells was measured by CCK-8 assay.Western blot assay was used to detect the expression of autophagy related protein LC3 and p62 in H9c2 cells in each group.RESULTS AND CONCLUSION:Compared with the blank control group,there was no significant change in cell survival rate in the sham operated rat serum group(P>0.05).Compared with the sham operated rat serum group,the cell survival rate(P<0.01)and the expression of LC3 protein(P<0.05)in spinal cord injury rat serum group was significantly reduced,and the expression of p62 protein was significantly increased(P<0.05).Compared with the spinal cord inju

关 键 词：脊髓损伤 血清 H9C2细胞 自噬 木犀草素 心功能不全 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R274