保肝颗粒对大鼠酒精性肝损伤的早期干预作用  

Early Intervention Effect of Baogan Granules on Alcoholic Liver Injury in Rats

作　　者：朱正望 高达 郭桓博 赵静涵 王琳琳 苗明三[2] 朱平生 ZHU Zheng-wang;GAO Da;GUO Huan-bo;ZHAO Jing-han;WANG Lin-lin;MIAO Ming-san;ZHU Ping-sheng(The First Clinical Medical College,Henan University of Chinese Medicine,Zhengzhou 450046,China;School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学第一临床医学院,郑州450046 [2]河南中医药大学药学院,郑州450046

出　　处：《科学技术与工程》2024年第12期4895-4901,共7页Science Technology and Engineering

基　　金：国家自然科学基金(82074340);河南省“双一流”创建学科中医学科学研究专项(HSRP-DFCTCM-2023-1-19,HSRP-DFCTCM-2023-8-32);河南省科技创新人才计划-杰出青年项目(154100510020);河南省高校科技创新团队支持计划项目(16IRTSTHN023)。

摘　　要：观察保肝颗粒对大鼠酒精性肝损伤的早期干预作用。将144只大鼠随机分为空白组、模型组、联苯双酯组、保肝颗粒低、中、高剂量组,给药1 h后除空白组外,其余各组按10 mL/kg灌胃50%乙醇,1 h后重复灌胃50%乙醇一次,空白组灌胃等体积蒸馏水,连续6 d。于实验开始的第2、3、4、6天每组分别随机处死大鼠6只,腹主动脉取血,检测血清中丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)、总胆红素(total bilirubin,TBIL)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)、α-谷胱甘肽-S-转移酶(α-glutathione S-transferase,α-GST)、谷氨酸脱氢酶(glutamate dehydrogenase,GLDH)、精氨酸酶1(arginase 1,Arg-1)、嘌呤核苷酸磷酸化酶(purine nucleotide phosphorylase,PNP)水平;肝匀浆中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)水平。结果表明:与空白组比,模型组大鼠血清AST、ALT、ALP、TBIL、TNF-α、IL-6、α-GST、GLDH、Arg-1、PNP水平明显升高(P<0.05,P<0.01),肝组织MDA水平明显升高、SOD水平明显降低(P<0.05,P<0.01);与模型组比,保肝颗粒可以明显降低血清AST、ALT、ALP、TBIL、TNF-α、IL-6、α-GST、GLDH、Arg-1、PNP的水平,提高肝组织中SOD的活性,降低MDA的含量(P<0.05,P<0.01)。说明保肝颗粒早期干预大鼠酒精性肝损伤有较好的保肝作用。To observe the early intervention effect of Baogan Granules on alcoholic liver injury in rats.144 rats were randomly divided into blank group,model group,biphenyl group,Baogan granules low,medium and high dose groups.After 1 h of administration,except for the blank group,the other groups were given 50%ethanol by gavage at 10 mL/kg,50%ethanol was gavaged repeatedly 1 h later,and the blank group was gavaged with the same volume of distilled water for 6 days.On the day 2,3,4 and 6 of the experiment,6 rats in each group were randomly killed,and blood was collected from abdominal aorta.The contents of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),total bilirubin(TBIL),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),α-glutathione S-transferase(α-GST),glutamate dehydrogenase(GLDH),arginase 1(Arg-1)and purine nucleotide phosphorylase(PNP)in serum and the levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in liver homogenate were determined.The results show that compared with blank group,the serum levels of AST,ALT,ALP,TBIL,TNF-α,IL-6,α-GST,GLDH,Arg-1 and PNP in model group are significantly increased(P<0.05,P<0.01),the level of MDA in liver tissue is significantly increased,and the level of SOD is significantly decreased(P<0.05,P<0.01).Compared with model group,Baogan Granules can significantly reduce the serum levels of AST,ALT,TBIL,ALP,TNF-α,IL-6,α-GST,GLDH,Arg-1,PNP(P<0.05,P<0.01).The activity of SOD in liver tissues increases and the content of MDA decreases(P<0.05,P<0.01).Baogan granules has a good effect on liver protection in the early intervention of alcoholic liver injury in rats.

关 键 词：保肝颗粒 酒精性肝病 大鼠 早期生物标志物 

分 类 号：R285.5[医药卫生—中药学]