芍药苷对慢性支气管炎模型大鼠Th17及Treg细胞亚群的影响  

Effect of paeoniflorin on Th17 and Treg cell subsets in chronic bronchitis model rats

作　　者：李修侠[1] 李玉玲 李传涛[1] LI Xiuxia;LI Yuling;LI Chuantao(Internal Medicine,Zaozhuang Maternal and Child Health Hospital,Zao-zhuang 277100,China;Outpatient Department,Zaozhuang Maternal and Child Health Hospital,Zaozhuang 277100,China)

机构地区：[1]枣庄市妇幼保健院内科,枣庄277100 [2]枣庄市妇幼保健院门诊,枣庄277100

出　　处：《中国免疫学杂志》2024年第4期786-790,共5页Chinese Journal of Immunology

摘　　要：目的:探究芍药苷对慢性支气管炎(CB)大鼠Th17、Treg细胞亚群、肺功能的影响以及药理作用。方法:将SD大鼠采用复合诱导法建立CB模型,随机分成CB组(生理盐水)、低剂量芍药苷组(50 mg/kg)、中剂量芍药苷组(100 mg/kg)、高剂量芍药苷组(200 mg/kg)和核酪组(阳性对照,核酪口服液),10只;另设对照组(生理盐水)10只,造模后按照分组灌胃2 ml/100 g相应药物。给药完成后,观察大鼠一般情况并进行肺功能及血气分析;HE染色观察肺组织形态;流式细胞仪检测大鼠肺组织CD4^(+)T、CD8^(+)T、Th17(CD4^(+)IL-17^(+)T)、Treg(CD4^(+)CD25^(+)FOXP3^(+)T)细胞;ELISA法检测肺组织中IL-10、IL-22、IL-17水平。结果:CB组大鼠活动迟缓,毛色暗淡,身体卷缩,伴咳嗽、鼻腔潮湿,肺组织可见淋巴细胞浸润、支气管壁变形、黏膜阻塞等病变;芍药苷各组及核酪组上述症状及损伤不同程度缓解,高剂量组最轻。与对照组比较,CB组气道阻力、动脉血二氧化碳分压(Pa‐CO_(2))、IL-17、IL-22水平及CD4^(+)T、CD8^(+)T、Th17细胞、Th17/Treg、CD4^(+)T/CD8^(+)T均升高,动脉血氧分压(PaO_(2))、IL-10水平及Treg细胞均降低(P均<0.05);与CB组比较,低、中、高剂量芍药苷组气道阻力、PaCO_(2)、IL-17、IL-22水平及CD4^(+)T、CD8^(+)T、Th17细胞、Th17/Treg、CD4^(+)T/CD8^(+)T水平依次降低,PaO_(2)、IL-10水平及Treg细胞依次升高(P均<0.05);中剂量芍药苷组气道阻力等以上指标与核酪组相比差异无统计学意义(P均>0.05)。结论:芍药苷可调节T淋巴细胞活化,减少Th17细胞分化,增加Treg细胞分化,减轻CB大鼠炎症水平及肺损伤。Objective:To explore the effects of paeoniflorin on Th17,Treg cell subsets and lung function in rats with chronic bronchitis(CB),so as to enrich its pharmacological effects.Methods:CB model was established by compound induction in SD rats,and SD rats were randomly divided into CB group(normal saline),low-dose paeoniflorin group(50 mg/kg),medium-dose paeoniflorin group(100 mg/kg),high-dose paeoniflorin group(200 mg/kg)and Helao group(positive control,Helao oral liquid),with 10 rats in each group;another 10 rats in the control group(normal saline)were given 2 ml/100 g corresponding drugs by gavage after modeling.After administration,the general conditions of rats were observed and the lung function and blood gas were analyzed;the morphology of lung tissue was observed by HE staining;the lung CD4^(+)T,CD8^(+)T,Th17(CD4^(+)IL-17^(+)T)and Treg(CD4^(+)CD25^(+)Foxp3^(+)T)cells were detected by flow cytometry;the levels of IL-10,IL-22 and IL-17 in the lung were detected by ELISA.Results:In CB group,the activity of rats were slow,the color of hair were dim,the body were curled up,accompanied with cough and moist nasal cavity,the above symptoms and injuries were alleviated in different degrees in paeoniflorin groups and Helao group,and the high dose group was the least.Compared with control group,the airway resistance,arterial partial pressure of carbon dioxide(PaCO_(2)),IL-17,IL-22 levels,CD4^(+)T,CD8^(+)T,Th17 cells,Th17/Treg,CD4^(+)T/CD8^(+)T were higher in CB group,arterial partial pressure of oxygen(PaO_(2)),IL-10 and Treg cells were lower(all P<0.05);compared with CB group,the airway resistance,PaCO_(2),IL-17,IL-22 levels,CD4^(+)T,CD8^(+)T,Th17 cells,Th17/Treg,CD4^(+)T/CD8^(+)T in low-,medium-and high-dose paeoniflorin groups decreased in turn,PaO_(2),IL-10 and Treg cells increased in turn(all P<0.05);there was no significant difference in airway resistance and the other above indexes between the medium-dose paeoniflorin group and Helao group(P>0.05).Conclusion:Paeoniflorin can regulate the activation of T lymphocyte

关 键 词：芍药苷 慢性支气管炎 Th17细胞 TREG细胞 炎症 

分 类 号：R285.5[医药卫生—中药学]