氯化钴模拟低氧条件下红景天苷对小鼠成骨细胞的调节作用  

Regulatory Effect of Salidroside on Mouse Osteoblasts Under Hypoxic Conditions Simulated by Cobalt Chloride

作　　者：罗婷 祁琳 李杰[1] 薛文舒 田晨晨 曹清文 王越 LUO Ting;QI Lin;LI Jie;XUE Wen-shu;TIAN Chen-chen;CAO Qing-wen;WANG Yue(Department of Stomatology,General Hospital of Tianjin Medical University,Tianjin 300052,China;Department of Pharmacy,Characteristic Medical Center of PAP,Tianjin 300162,China;School of Integrative Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津医科大学总医院口腔科,天津300052 [2]武警特色医学中心药剂科,天津300162 [3]天津中医药大学中西医结合学院,天津301617

出　　处：《医学信息》2024年第9期101-106,共6页Journal of Medical Information

基　　金：天津伊特生命科学研发有限公司项目(编号:HX2023-7);天津市自然科学基金重点项目(编号:18JCZDJC33200);天津中医药大学研究生创新基金(编号:ZXYCXLX202119,ZXYCXLX202213)。

摘　　要：目的研究氯化钴(CoCl_(2))模拟的低氧条件下红景天苷(SAL)对小鼠成骨细胞的调节作用。方法用CoCl_(2)模拟低氧条件(1%O_(2)),建立体外低氧模型。采用MTS法研究低氧条件下不同浓度SAL对MC3T3-E1细胞增殖的影响,磷酸苯二钠法研究低氧条件下SAL对MC3T3-E1细胞分化的影响,实时荧光定量PCR(RT-qPCR)、Westernblot及ELISA技术检测低氧诱导因子-1α(HIF-1α)、下游靶基因血管内皮生长因子(VEGF)mRNA及蛋白表达水平。结果确定用0.5mmol/L的CoCl_(2)来模拟低氧(1%O_(2))环境。低氧可明显抑制MC3T3-E1细胞的增殖及分化。经SAL预处理后,可显著促进MC3T3-E1细胞的增殖及分化,其中SAL(10 nmol/L)可显著上调HIF-1α和VEGF的mRNA表达水平及HIF-1α的蛋白表达水平,并下调VEGF蛋白的表达。结论低氧条件下SAL促进小鼠成骨细胞增殖与分化。其具体机制有待进一步研究,可能与HIF-1α/VEGF信号通路有关。Objective To study the regulatory effect of salidroside(SAL)on mouse osteoblasts under hypoxic conditions simulated by cobalt chloride(CoCl_(2)).Methods CoCl_(2)was used to simulate hypoxic conditions(1%O_(2))to establish an in vitro hypoxic model.MTS method was used to study the effect of different concentrations of SAL on the proliferation of MC3T3-E1 cells under hypoxic conditions.The effect of SAL on the differentiation of MC3T3-E1 cells under hypoxic conditions was studied by disodium phenyl phosphate method.Real-time fluorescence quantitative PCR(RT-qPCR),Western blot and ELISA were used to detect the mRNA and protein expression levels of hypoxia-inducible factor-lα(HIF-la)and downs.Results 0.5 mmol/L CoCl_(2)was used to simulate the hypoxic(1%O_(2))environment.Hypoxia could significantly inhibit the proliferation and differentiation of MC3T3-E1 cells.After pretreatment with SAL,the proliferation and differentiation of MC3T3-E1 cells were significantly promoted.SAL(10 nmol/L)significantly up-regulated the mRNA expression levels of HIF-1αand VEGF and the protein expression level of HIF-Iα,and down-regulated the expression of VEGF protein.Conclusion SAL promotes the proliferation and differentiation of mouse osteoblasts under hypoxia.The specific mechanism needs to be further studied,which may be related to the HIF-1α/VEGF signaling pathway.

关 键 词：红景天苷 成骨细胞 增殖 分化 骨结合 HIF-1α/VEGF通路 

分 类 号：R285.5[医药卫生—中药学]