河南省番茄灰霉病病原菌鉴定、生物学特性分析及杀菌剂筛选  

Identification,Biological Characteristics Analysis and Fungicide Screening for Pathogenic Pathogen Causing Tomato Gray Mold in Henan Province

作　　者：杨囡君[1] YANG Nanjun(Shangqiu Vocational and Technical College,Shangqiu,Henan 476100,China)

机构地区：[1]商丘职业技术学院,河南商丘476100

出　　处：《陕西农业科学》2024年第2期33-38,97,共7页Shaanxi Journal of Agricultural Sciences

基　　金：河南省高等学校重点科研项目支撑计划(编号:23B2120006)生物农药发展及在作物生产中的应用研究。

摘　　要：番茄灰霉病是番茄生产上的重要病害之一。本试验以河南商丘市蔬菜大棚内疑似患灰霉病番茄植株叶片为试材,采用组织分离法纯化获得3株病原菌菌株,其形态特征一致,选择一株代表性菌株命名为SQBC1。对该菌株rDNA-ITS序列、CHS-1、tubA基因进行测序、比对和系统进化分析,结合菌株培养性状、显微形态学观察、致病性分析,将河南商丘番茄灰霉病致病病原菌确定为灰葡萄孢菌Botryotinia cinerea。生物学特性研究表明28℃为最适生长温度,12 h光照12 h黑暗为最适光照条件,6.0为最适pH值,乳糖为最适碳源,蛋白胨为最适氮源。测试了7种杀菌剂对灰霉菌菌丝生长的抑菌效果,其中嘧菌酯和吡唑醚菌酯的抑菌效果最好。该研究为番茄灰霉病的有效防治提供一定的参考依据。Gray mold of tomato is one of the serious diseases affecting tomato production.The tomato leaves infected with gray mold from vegetable greenhouses in Shangqiu,Henan Province was used as material.Three pathogen strains were purified by tissue isolation method,and their morphological characteristics were found to be identical.One representative strain was named as SQBC1.The rDNA-ITS,CHS-1 gene and tubA gene of the strain were sequenced.In combination with culture characteristic,morphological characteristics,and pathogenicity,the pathogen causing tomato gray mold in Shangqiu City,Henan Province was identifed as Botrytis cinerea.The strain’s optimal growth temperature was 28℃,with the ideal light conditions being 12 hours of daylight and 12 hours of darkness.The optimal pH was 6.0,while lactose was the preferred carbon source,and peptone was the optimal nitrogen source.The inhibitory effects on the the growth of the pathogen tomato gray mold were assessed,indicating significant inhibitory effects of azoxystrobin and pyraclostrobin.The study provides a reference for effective control of tomato gray mold.

关 键 词：番茄 灰霉病 灰葡萄孢菌 致病性鉴定 ITS序列 

分 类 号：S641.3[农业科学—蔬菜学]