小叶黄杨叶中化学成分对非小细胞肺癌A549增殖抑制作用的研究  

The Chemical Components in the Leaves of Buxus Microphylla on the Proliferation of Non-small Cell Lung Cancer A549 Research on Inhibitory Effects

作　　者：明洋 赵莎 郑一敏[1] 胥秀英[1] MING Yang;ZHAO Sha;ZHENG Yi-min;XU Xiu-ying(Chongqing University of Technology,School of Pharmacy and Bioengineering,Chongqing 400050,China)

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400050

出　　处：《宜春学院学报》2024年第3期20-23,113,共5页Journal of Yichun University

摘　　要：目的:研究小叶黄杨叶中的化学成分抑制非小细胞肺癌A549增殖作用。方法:通过回流提取、萃取、硅胶色谱柱与质谱、核磁等方法进行分离纯化及结构确证。采用CCK8法,对分离得到化合物进行非小细胞肺癌A549体外活性评价。结果:从小叶黄杨叶中分离得到8个化合物,鉴定为白桦脂醇(1)、羽扇豆醇(2)、羟基环原黄杨碱(3)、羟基环原黄杨碱酯(4)、N-甲基黄杨宁(5)、苦参素(6)、3-氨基-4-(羟基甲基)-4,14-二甲基-9,19-环丙基-17-二甲基氨基-23-苯甲酰酯甾烷(7)、3-氨基-4,14-二甲基-9,19-环丙基-16-甲基丁酸甲酯-17-二甲基氨基-23-苯甲酰酯甾烷(8)。活性结果表明化合物1-8均能抑制非小细胞肺癌A549增殖。结论:化合物5、7、8为首次从小叶黄杨叶中分离得到的新化合物;苦参素(6)为首次从小叶黄杨中叶分离得到。化合物7抗非小细胞肺癌A549作用最强。Objective:To study the inhibitory effect of chemical components in the leaves of buxus microphylla on the proliferation of non-small cell lung cancer A549.Methods:Separation,purification,and structural confirmation were carried out through reflux extraction,extraction,silica gel chromatography column and mass spectrometry,nuclear magnetic resonance,and other methods.Using the CCK8 method,evaluate the in vitro activity of the isolated compound for non-small cell lung cancer A549.Results:Eight compounds were isolated from the leaves of small leaved boxwood and identified as betulin alcohol(1),lupin alcohol(2),hydroxycyclo boxwood alkaloid(3),hydroxy cyclo boxwood alkaloid ester(4),N-methyl boxwood tannin(5),matrine(6),3-amino-4-(hydroxymethyl)-4,14-dimethyl-9,19-cyclopropyl-17-dimethylamino-23-benzoyl ester sterane(7),3-amino-4,14-dimethyl-9,19-cyclopropyl-16-methylbutyrate-methyl-17-dimethylamino-23-benzoyl ester sterane(8).The activity results indicate that compounds 1-8 can inhibit the proliferation of non-small cell lung cancer A549.Conclusion:Compounds 5,7,and 8 are new compounds isolated from the small leaved yellow poplar for the first time.Oxymatrine(6)was isolated from Buxus Microphylla for the first time.Compound 7 has the strongest anti non-small cell lung cancer effect on A549.

关 键 词：小叶黄杨叶 非小细胞肺癌 增殖抑制 

分 类 号：R917[医药卫生—药物分析学]