胎盘ULK1表达与自噬及子痫前期的相关性研究  

Correlation between the expression of ULK1 in the placenta and autophagy as well as preeclampsia

作　　者：葛榕[1] 陈品玲 郑琳[1] 杨茵[1] 林靓[1] 辛长征 李蕊[1] 许昌声 GE Rong;CHEN Pin-ling;ZHENG Lin;YANG Yin;LIN Jing;XIN Chang-zheng;LI Rui;XU Chang-sheng(Department of Gynaecology and Obstetrics,Fujian Provincial Hospital,Shengli Clinical Medical College of Fujian Medical University,Fuzhou 350027,Fujian,CHINA;Fujian Provincial Hypertension Research Institute,Fuzhou 350001,Fujian,CHINA)

机构地区：[1]福建医科大学省立临床医学院福建省立医院妇产科,福建福州350027 [2]福建省高血压研究所,福建福州350001

出　　处：《海南医学》2024年第9期1283-1286,共4页Hainan Medical Journal

基　　金：福建省自然科学基金资助项目(编号:2020J011102)。

摘　　要：目的比较子痫前期与正常产妇胎盘组织中Unc-51样激酶1(ULK1)表达和自噬水平,分析胎盘ULK1表达与自噬水平及子痫前期病情的相关性。方法选择2021年3月至2023年5月于福建省立医院分娩的25例子痫前期患者作为子痫前期组,同期住院且年龄、产次与分娩方式相配对的25例正常产妇作为对照组。免疫组织化学检测两组产妇胎盘组织中自噬标志蛋白微管相关蛋白1A/1B-轻链3B(LC3B)、苄氯素1(Beclin1)、螯合体1(SQSTM1/p62)和ULK1蛋白的表达水平,实时荧光定量PCR检测胎盘组织ULK1的mRNA表达,电化学发光法检测子痫前期患者外周血可溶性类fms酪氨酸激酶-1(sFlt-1)和胎盘生长因子(PlGF),采用Pearson法分析ULK1表达与sFlt-1/PlGF比值的相关性。结果免疫组织化学检测结果显示,对照组与子痫前期组胎盘均可见LC3B、Beclin1和p62表达,经半定量分析结果显示,子痫前期组产妇胎盘组织的LC3B和Beclin1表达相对量分别为695.32±77.82、534.19±77.60,明显高于对照组的630.11±64.14和438.66±91.71,p62表达相对量为529.66±85.62,明显低于对照组的638.37±127.62,差异均有统计学意义(P<0.05);经实时荧光定量PCR检测结果显示,子痫前期组产妇胎盘组织ULK1的mRNA表达水平1.01±0.13,明显高于对照组的0.86±0.08,差异有统计学意义(P<0.01);子痫前期组外周血PlGF和sFlt-1水平分别为(114.12±21.71)pg/mL和(8274.126±3453.49)pg/mL,sFlt-1/PlGF比值平均为81.94±57.84,经Pearson相关性分析表明,子痫前期组胎盘组织ULK1的mRNA表达水平与外周血sFlt-1/PlGF比值呈正相关(r=0.701,P<0.05)。结论子痫前期患者胎盘组织ULK1的表达水平明显上升,与自噬水平一致,并与子痫前期严重程度密切相关,有望作为子痫前期防治的新靶点。Objective To compare the expression of Unc-51-like kinase 1(ULK1)and autophagy levels in the placenta between preeclampsia and normal postpartum women,and to analyze the correlation between ULK1 expression and autophagy levels in the placenta as well as the condition of preeclampsia.Methods A total of 25 preeclampsia patients who delivered in Fujian Provincial Hospital from March 2021 to May 2023 were selected as preeclampsia group.Another 25 normal parturients who were hospitalized during the same period and matched in age and delivery methods were selected as the control group.The protein expression levels of microtubule-associated protein light-chain 3B(LC3B),benzyl chloride 1(Beclin1),sequestosome 1(SQSTM1/p62),and ULK1 in the placenta were detected by immunohistochemistry.The mRNA expression of ULK1 in the placentas was detected by real-time fluorescence quantitative PCR.The soluble fms tyrosine kinase-1(sFlt-1)and placental growth factor(PlGF)levels in the peripheral blood of the preeclampsia patients were detected by electrochemiluminescence,and the correlation between ULK1 expression and sFlt-1/PlGF ratio was analyzed by Pearson correlation analysis.Results Immunohistochemical test showed that LC3B,Beclin1,and p62 were expressed in the placentas in both the control group and the preeclampsia group.Semi-quantitative analysis showed that the relative expression levels of LC3B and Beclin1 in the placental tissue of the preeclampsia group were 695.32±77.82 and 534.19±77.60,respectively,which were significantly higher than 630.11±64.14 and 438.66±91.71 of the control group(P<0.05).The relative expression of p62 in the placental tissue of the preeclampsia group was significantly lower than that in the control group:(529.66±85.62)vs(638.37±127.62),P<0.05.The results of real-time fluorescence quantitative PCR showed that the mRNA expression level of ULK1 in the placental tissue of the preeclampsia group was significantly higher than that in the control group:(1.01±0.13)vs(0.86±0.08),P<0.05.In the preeclam

关 键 词：子痫前期 自噬 Unc-51样激酶1 胎盘 

分 类 号：R714.245[医药卫生—妇产科学]