SND1在食管鳞癌细胞中的表达及对食管癌细胞恶行生物学行为的影响  

Expression of SND1 in esophageal squamous cell carcinoma and its effect on malignant biological behavior of esophageal carcinoma cells

作　　者：尹辉 吴剑 胡智 黄山 闫群伦 戴天阳 YIN Hui;WU Jian;HU Zhi;HUANG Shan;YAN Qunlun;DAI Tianyang(Thoracic Surgery,The Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan,China)

机构地区：[1]西南医科大学附属医院胸外科,四川泸州646000

出　　处：《西部医学》2024年第5期674-680,共7页Medical Journal of West China

基　　金：四川省科技厅项目(2022NSFSC0768)。

摘　　要：目的研究葡萄球菌核酸酶结构域蛋白1(SND1)基因在人食管鳞状细胞癌(ESCC)中表达水平的表达及对食管癌细胞恶行生物学行为的影响。方法qRT-PCR及Western blot检测SND1在ESCC细胞株中翻译和转录水平的改变。运用sh-SND1病毒载体构建出SND1稳定干扰的ESCC细胞株模型,利用克隆形成,Transwell小室及流式细胞术等细胞生物学手段研究SND1基因对ESCC细胞增殖和转移、凋亡的影响,运用动物实验研究SND1基因在体内对ESCC细胞的影响。结果qRT-PCR和Western blot的实验结果表明SND1在ESCC细胞中的转录和翻译水平高于人正常食管上皮细胞(HEEC);经过培养、染色、拍照和通过统计学分析细胞表型实验数据,结果显示干扰SND1基因表达可显著抑制ESCC细胞的增殖、迁移和侵袭能力;干扰SND1的表达抑制体内食管癌细胞的增殖能力;化疗药物敏感实验结果显示SND1干扰组食管癌细胞对CPT的敏感性增加,细胞凋亡率明显升高。结论SND1在食管癌细胞中表达上调及促进食管癌细胞恶性生物学行为。Objective To study the expression level of SND1 gene in human esophageal squamous cell carcinoma and its effect on malignant biological behavior of esophageal cancer cells.Methods qRT-PCR and Western blot were used to detect the changes of translation and transcription levels of SND1 in ESCC cell lines.The ESCC cell line model with stable interference of SND1 was constructed by sh-SND1 virus vector,and the effects of SND1 gene on the proliferation,metastasis and apoptosis of ESCC cells were studied by cell biology methods such as clone formation,Transwell chamber and flow cytometry.The effect of SND1 gene on ESCC cells in vivo was studied by animal experiments.Results The results of qRT-PCR and Western blot showed that the transcription and translation level of SND1 in ESCC cells was higher than that in normal human esophageal epithelial cells.After culture,dyeing,photographing and statistical analysis of cell phenotype experimental data,the results show that interfering with SND1 gene expression can significantly inhibit the proliferation,migration and invasion of ESCC cells.Interference with SND1 expression inhibits the proliferation of esophageal cancer cells in vivo.The results of chemosensitivity test showed that the sensitivity of esophageal cancer cells to CPT increased and the apoptosis rate increased significantly in SND1 interference group.Conclusion The expression of SND1 is up-regulated in esophageal cancer cells and promotes the malignant biological behavior of esophageal cancer cells.

关 键 词：SND1 食管癌 增殖 迁移 侵袭 凋亡 

分 类 号：R655.4[医药卫生—外科学]