蛇含委陵菜抗炎活性部位的筛选及化学成分研究  

Screening of anti-inflammatory fractions and chemical constituents from Potentilla kleiniana Wight.et Arn.

作　　者：赵珊 孙绪[3] 刘佳 周艳 匡维米[4] 金倩倩 张宝[1,2] 李悦 ZHAO Shan;SUN Xu;LIU Jia;ZHOU Yan;KUANG Weimi;JIN Qianqian;ZHANG Bao;LI Yue(Department of Pharmacy,Guiyang Maternal and Child Health Hospital/Guiyang Children’s Hospital,Guiyang 550003,China;College of Women and Children Clinical Medicine,Guizhou Medical University,Guiyang 550003,China;Guizhou Provincial Institute for Food and Drug Control,Guiyang 550004,China;Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵阳市妇幼保健院/贵阳市儿童医院药学部,贵阳550003 [2]贵州医科大学妇幼临床学院,贵阳550003 [3]贵州省食品药品检验所,贵阳550004 [4]贵州医科大学贵州省药物制剂重点实验室,贵阳550004

出　　处：《西北药学杂志》2024年第3期10-15,共6页Northwest Pharmaceutical Journal

基　　金：贵州省中医药、民族医药科学技术研究专项(编号:QZYY-2021-176);贵阳市卫生健康局高层次创新型青年卫生人才培养计划项目(编号:【2022】筑卫健科技合同字第010号)。

摘　　要：目的研究蛇含委陵菜的抗炎活性部位及化学成分。方法用二甲苯致小鼠耳肿胀急性炎症模型筛选蛇含委陵菜体积分数为60%的乙醇提取物的水部位(FPA)、体积分数为50%的乙醇部位(FPB)、体积分数为95%的乙醇部位(FPC)的抗炎活性。进一步用MCI、Sephadex LH-20、正相和反相硅胶等柱层析方法对活性部位的化学成分进行分离纯化,通过理化性质和核磁共振波谱对分离得到的化合物进行结构鉴定。结果蛇含委陵菜FPB具有抗炎活性,并从该部位中分离得到10个化合物,分别鉴定为:补骨脂素(1)、邻苯二甲酸-双(2’-乙基庚基)酯(2)、毛两面针素(3)、附子亭碱(4)、1,2-二羟基蒽醌(5)、熊果苷(6)、山柰酚(7)、桔皮素(8)、异鼠李素(9)、金合欢素-7-O-α-L-吡喃鼠李糖-(1→6)-β-D-吡喃葡萄糖(10)。结论蛇含委陵菜FPB是其抗炎活性部位;从该部位中分离鉴定了10个化合物,其中化合物2~4、7~10为首次从委陵菜属植物中分离得到,化合物1、6为首次从该植物中分离得到。Objective To screen the anti-inflammatory fractions of Potentilla kleiniana Wight.et Arn.and to study the chemical constituents from the active fractions.Methods The anti-inflammatory activity of water fraction(FPA),50%ethanol fraction(FPB)and 95%ethanol fraction(FPC)of 60%ethanol extract Potentilla kleiniana Wight.et Arn.were evaluated by mice ear swelling acute inflammation model induced by xylene.The constituents from active fraction were further isolated by MCI,Sephadex LH-20,silica gel,octadecyl-silylated silica gel column chromatography and other methods,and their chemical structures were elucidated on the basis of physicochemical properties and spectral analysis.Results The 50%ethanol fraction exhibited anti-inflammatory activity,and 10 compounds were isolated and their structures were identified as:psoralen(1),bis(2’-ethylheptyl)phythalate(2),toddalolactone(3),fuzitine(4),1,2-oxyanthraquinone(5),arbutin(6),kaemperol(7),tangeretin(8),isorhamnetin(9),and acacetin-7-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside(10).Conclusion The 50%ethanol fraction is the antiinflammatory fraction,from which 10 compounds were separated and elucidated.Compouds 2—4,7—10 were isolated from Potentilla genus for the first time,and compounds 1 and 6 were isolated from Potentilla kleiniana Wight.et Arn.for the first time.

关 键 词：蛇含委陵菜 化学成分 结构鉴定 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]