多指标综合评分法结合响应曲面法筛选蜜炙款冬花的最佳炮制工艺  

Optimization of the processing technology of honey baked Farfarae Flos by response surface methodology experimental design with multi-index

作　　者：于上林 吴璐叶 YU Shanglin;WU Luye(Department of Pharmacy,the 900th Hospital of the Joint Logistics Support Force of Chinese PLA,Fuzhou 350000,China)

机构地区：[1]中国人民解放军联勤保障部队第九〇〇医院药剂科,福州350000

出　　处：《西北药学杂志》2024年第3期16-20,共5页Northwest Pharmaceutical Journal

摘　　要：目的基于响应曲面法,从多指标综合评价的角度建立蜜炙款冬花的最佳炮制工艺。方法用高效液相色谱(high performance liquid chromatography,HPLC)定量分析,以款冬酮、绿原酸、异绿原酸A和芦丁含量的综合评分值为考察指标,以加蜜量、炒制温度、炒制时间为单因素,用响应曲面法优选出蜜款冬花的最优炮制工艺并进行验证实验。结果优选的蜜炙款冬花最佳炮制工艺为加蜜量为4倍药材量,炒制温度为195℃,炒制时间为12 min。验证实验中3批样品提取后的综合评分值(94.16%)与预测值(95.92%)相比,相对误差为0.93%。结论该实验的方法学考察结果准确、重复性好,用响应曲面法优选的蜜炙款冬花的最佳炮制方案可行,为制定蜜炙款冬花的质量标准提供了理论依据。Objective Based on response surface methodology,the optimal processing technology of honey baked Farfarae Flos was established from the point of multi-index evaluation.Methods High performance liquid chromatography(HPLC)was used for quantitative analysis,and the content of tussilagone,chlorogenic acid,isochlorogenic acid A and rutin was used as inspection indexes.Response surface methodology was used to investigate the effects of the adding amount of honey,frying temperature and frying time on the processing technology of honey baked Farfarae Flos,and to optimize the processing technology of honey baked Farfarae Flos.Results The optimal processing process of honey baked Farfarae Flos was as follows:the amount of honey was 4 times the amount of medicinal materials,frying pan bottom temperature was 195℃,and frying time was 12 minutes.In validation test,the relative deviation of comprehensive scoring value(94.16%)to predicted value(95.92%)was 0.93%for 3 batches of samples(n=3).Conclusion The results of this experiment are accurate and repeatable,and the optimal processing scheme of honey baked Farfarae Flos is feasible,providing a theoretical basis for formulating quality standards for honey baked Farfarae Flos.

关 键 词：蜜款冬花 高效液相色谱法 炮制工艺 响应曲面法 款冬酮 绿原酸 异绿原酸A 芦丁 

分 类 号：R282[医药卫生—中药学]