PEG降低HIV第四代试剂检测假阳性干扰的研究  

Study on PEG reducing false positive interference in HIV fourth generation reagent detection

作　　者：阳冬冬 穆银玉[1] 张日伊 李静 徐琳 YANG Dongdong;MU Yinyu;ZHANG Riyi;LI Jing;XU Lin(Clinical Laboratory,Ningbo Medical Center,Lihuili Hospital,Ningbo 315040,Zhejiang,China)

机构地区：[1]宁波市医疗中心李惠利医院检验科,浙江宁波315040

出　　处：《中国现代医生》2024年第13期40-42,59,共4页China Modern Doctor

摘　　要：目的探讨聚乙二醇(polyethylene glycol,PEG)沉淀法降低化学发光法人类免疫缺陷病毒(human immunodeficiency virus,HIV)第四代试剂检测假阳性干扰的研究。方法选取2022年1月至6月就诊于宁波市医疗中心李惠利医院的门诊和住院患者109例,经化学发光法初筛阳性的样本经蛋白质印迹法确证实验,确证真阳性样本20例、假阳性样本89例。根据是否添加处理剂将假阳性样本分为三组:无PEG沉淀组(无特殊处理,89例)、PEG沉淀组(PEG沉淀处理,89例)和对照组(生理盐水处理,89例),离心后测定上清液HIV抗原/抗体(antigen/antibody,Ag/Ab)水平,比较不同处理方法前后HIV Ag/Ab水平的差异及回收率。结果处理后,PEG沉淀组和对照组的HIV Ag/Ab浓度显著均低于无PEG沉淀组(P<0.001)。处理后,PEG沉淀组阳性3例,对照组阳性56例,阳性率均显著低于无PEG沉淀组(P<0.001);PEG沉淀组的回收率为4.92(2.12,12.69)%,对照组的回收率为65.28(18.04,91.28)%。不论PEG处理还是生理盐水处理,真阳性样本的回收率均>50%。结论PEG沉淀法可有效降低化学发光法HIV第四代试剂检测的假阳性干扰,且对真阳性样本无影响。Objective Study on the reduction of false positive interference in the detection of human immunodeficiency virus(HIV)by chemiluminescence method with polyethylene glycol(PEG)precipitation.Methods A total of 109 outpatients and inpatients in Ningbo Medical Center,Lihuili Hospital from January to June 2022 were selected.The positive samples initially screened by chemiluminescence method were confirmed by Western blotting,and 20 true positive samples and 89 false positive samples were confirmed.According to whether treatment agent was added,the false-positive samples were divided into three groups:no PEG precipitation group(no special treatment,89 cases),PEG precipitation group(PEG precipitation treatment,89 cases)and control group(normal saline treatment,89 cases).The level of HIV antigen/antibody(Ag/Ab)in supernatant was measured after centrifugation,and the difference of HIV Ag/Ab level and recovery rate before and after different treatment methods were compared.Results After intervention,the HIV Ag/Ab concentration in PEG precipitation group and control group was significantly lower than that in non-PEG precipitation group(P<0.001).After intervention,there were 3 positive cases in PEG precipitation group and 56 positive cases in control group,and the positive rates were significantly lower than those in non-PEG precipitation group(P<0.001).The recovery rate of PEG precipitation group was 4.92(2.12,12.69)%,and that of control group was 65.28(18.04,91.28)%.The recovery rate of true positive samples was>50%regardless of PEG treatment or normal saline treatment.Conclusion PEG precipitation method can effectively reduce the false positive interference of chemiluminescent HIV fourth-generation reagent detection,and has no effect on true positive samples.

关 键 词：人类免疫缺陷病毒 聚乙二醇 化学发光法 假阳性 干扰 

分 类 号：R446.1[医药卫生—诊断学]