基于网络药理学结合UHPLC-MS/MS探讨清感童饮的抗炎作用  

The Anti-inflammatory Effects of Qinggan Tongyin Based on the Network Pharmacology and UHPLC-MS/MS

作　　者：呼延皓冉 王丽雯 张孝莹 张玥[1,2,3] 周昆[1,2,3] HUYAN Haoran;WANG Liwen;ZHANG GXiaoying;ZHANG Yue;ZHOU Kun(Institute of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;State Key Laboratory of Component-based Chinese Medicine,Tianjin 301617,China;Centre of Drug Safety Evaluation,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学中医药研究院,天津301617 [2]天津中医药大学组分中药国家重点实验室,天津301617 [3]天津中医药大学药物安全评价中心,天津301617

出　　处：《中药新药与临床药理》2024年第3期368-375,共8页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：天津市中医药重点领域科研项目(2021004)。

摘　　要：目的 基于血清药理学和网络药理学探究清感童饮的体外抗炎作用。方法 采用血清药理学评价方法,验证清感童饮含药血清对脂多糖(LPS)诱导的小鼠巨噬细胞(RAW264.7)释放一氧化氮(NO)、细胞坏死因子α(TNF-α)、白细胞介素6(IL-6)的影响。利用液质联用(UHPLC-MS/MS)技术对清感童饮指标性成分进行含量测定,并根据这些成分进行网络药理学分析,对清感童饮有效成分发挥抗炎作用的潜在靶点及作用通路进行预测。结果 细胞实验证明清感童饮可明显降低NO、TNF-α、IL-6水平(P<0.05,P<0.01,P<0.001)。清感童饮中含量较高的成分为连翘酯苷A、牛蒡苷、绿原酸、野黄芩苷、没食子酸、迷迭香酸、芍药内酯苷、连翘苷。清感童饮中17个活性成分与炎症的交集靶点共215个,主要涉及ALB、VEGFA、IL-6、TNF-α等31个核心靶点,调节AGE-RAGE、PI3K-Akt、MAPK信号通路等多种通路发挥抗炎作用。结论 清感童饮具有多成分-多靶点发挥抗炎作用的特点。Objective To preliminary explore the in vitro anti-inflammatory effects of Qinggan Tongyin based on serum pharmacology and network pharmacology.Methods The effects of the serum containing Qinggan Tongyin on the release of NO,cell necrosis factor-α(TNF-α),and interleukin-6(IL-6)in LPS-induced RAW264.7 cells were confirmed using serum pharmacology.UHPLC-MS/MS was used to determine the index components of Qinggan Tongyin.The possible targets and pathways of active components in Qinggan Tongyin for anti-inflammatory properties were predicted by using network pharmacology.Results The results of cellular assay showed that Qinggan Tongyin could dramatically lessen the levels of NO,TNF-α,and IL-6(P<0.05,P<0.01,P<0.001).The higher contents of Qinggan Tongyin were phillyrin A,arctiin,chlorogenic acid,scutellarin,gallic acid,rosmarinic acid,paeoniflorin and phillyrin.A totsl of 215 intersection targets between 17 active components in Qinggan Tongyin and inflammation were obtained,and the 31 core targets were ALB,VEGFA,IL-6,TNF-α,etc..The primary targets can exhibit anti-inflammatory actions by regulating several signaling pathways,such as AGE-RAGE,PI3K-Akt,and MAPK signaling pathway.Conclusion Qinggan Tongyin exerts its anti-inflammatory effects with the characteristic of multiple components and multiple targets.

关 键 词：清感童饮 抗炎作用 血清药理学 网络药理学 UHPLC-MS/MS 小鼠巨噬细胞(RAW264.7) 小鼠 

分 类 号：R285.5[医药卫生—中药学]