EMS诱变菜豆子叶节定向筛选耐羟脯氨酸(HYP)突变体  

EMS Mutagenic Phaseolus vulgaris L. Cotyledonary Nodes Targeted Screening of Hydroxyproline-resistant (HYP) Mutants

作　　者：许莹楠 刘艳军 黄俊轩 韩启厚[2,3] 古瑜 高逸卓[1] XU Yingnan;LIU Yanjun;HUANG Junxuan;HAN Qihou;GU Yu;GAO Yizhuo(Tianjin Agronomy College,Tianjin 300392;Tianjin Kernel Agricultural Science and Technology Co.Ltd.Vegetable Research Institute/State Key Laboratory of Vegetable Germplasm Innovation/Tianjin Key Laboratory of Vegetable Breeding Enterprise;Tianjin Academy of Agricultural Sciences)

机构地区：[1]天津农学院园艺园林学院,天津300392 [2]天津科润农业科技股份有限公司蔬菜研究所/蔬菜种质创新国家重点实验室/天津市蔬菜遗传育种企业重点实验室 [3]天津市农业科学院

出　　处：《长江蔬菜》2024年第10期56-59,共4页Journal of Changjiang Vegetables

基　　金：天津市科技重大专项与工程计划项目“荚用菜豆新种质创制方法及种质资源创新研究”(合同编号:17ZXZYNC00030);2022年天津市财政种业创新专项“豆类蔬菜育种技术创新及新品种育繁推一体化研究”(合同编号:2022ZYCX003);2023年天津市财政种业创新专项“花椰菜、芹菜等优势蔬菜作物育种技术创新与新品种选育”(合同编号:2023ZYCX010)。

摘　　要：以双丰一号菜豆子叶节为外植体,利用化学诱变剂EMS处理引发基因突变,进行突变体定向筛选抗逆突变细胞系。以0.17%EMS诱变处理2 h,子叶节的不再生率接近50%;以羟脯氨酸最大致死量3.5 mmol/L为筛选压力,定向筛选抗逆突变体。结果显示,在羟脯氨酸筛选培养基上,经EMS诱变的子叶节存活31个,突变率为8.61%,存活的子叶节再生率为74.19%,成功获得耐羟脯氨酸的突变株系。The mutagen EMS was used to induce the gene mutation,and the mutant was induced by mutagenesis technology to purposefully screen the stress-resistant mutant cell lines with the explants of Shuangfeng No.1 Phaseolus vulgaris L.cotyledonary nodes.The mutagenic concentration of EMS was 0.17%,and the non-regeneration rate of cotyledonary nodes was close to 50%after treatment with 0.17%EMS for 2 h.The maximum lethal dose of hydroxyproline 3.5 mmol/L was used as the screening pressure to screen the stress-resistant mutants.The results showed that 31 cotyledonary nodes survived after EMS mutagenesis on the hydroxyproline screening medium,with the mutation rate of 8.61%and the regeneration rate of 74.19%,which indicated that the mutant line resistant to hydroxyproline was successfully obtained.

关 键 词：EMS诱变 羟脯氨酸 抗逆突变体 

分 类 号：S643.1[农业科学—蔬菜学]