表没食子儿茶素没食子酸酯与氟康唑联用抗耐药白色念珠菌的协同作用及机制  

Synergistic effect and mechanism of epigallocatechin gallate and fluconazole against Candida albicans resistant isolates

作　　者：张柳平 李灿 李秀云 王菲菲 ZHANG Liuping;LI Can;LI Xiuyun;WANG Feifei(Department of Pharmacy,Shanxian Central Hospital,Heze 274300,China;Shandong Provincial Maternal and Child Health Care Hospital,Jinan 250014,China;Weifang No.2 People′s Hospital,Weifang 261041,China)

机构地区：[1]单县中心医院药剂科,山东菏泽274300 [2]山东省妇幼保健院,山东济南250014 [3]潍坊市第二人民医院,山东潍坊261041

出　　处：《药学研究》2024年第4期338-341,共4页Journal of Pharmaceutical Research

基　　金：山东省自然科学基金(No.ZR2020QH365)。

摘　　要：目的探讨表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)与氟康唑(fluconazole,FLC)联用对耐药白色念珠菌(Candida albicans,CA)的协同抗感染效果及作用机制。方法选用6株耐药CA,通过棋盘微量稀释试验,利用部分抑菌浓度指数(fractional inhibitory concentration index,FICI)评价联合作用的抗耐药CA作用;及利用荧光显微镜观察两药联用对菌丝生长的影响。结果通过FICI评价法,证实了EGCG与氟康唑联用具有协同抗耐药CA作用,且二者的协同作用可能与抑制菌丝生长有关。结论EGCG与FLC联用具有协同抗耐药CA的作用,且协同作用机制可能与抑制菌丝生长有关。Objective To investigate the synergistic anti-infection effect of epigallocatechin gallate(EGCG)combined with fluconazole on drug-resistant CA and its mechanism of action.Methods Six strains of drug-resistant CA were selected and evaluated by checkerboard microdilution test with fractional inhibitory concentration index(FICI).The effect of the combination of the two drugs on the growth of mycelia was observed by fluorescence microscope.Results By FICI evaluation,it was confirmed that EGCG combined with FLC had a very good synergistic action against drug resistant CA,and the synergistic effect of the two was probably related to the inhibition of mycelial growth.Conclusion EGCG combined with FLC has synergistic action against drug resistant CA,and the synergistic mechanism may be related to inhibition of mycelial growth.

关 键 词：耐药白色念珠菌 氟康唑 表没食子儿茶素没食子酸酯 协同作用 菌丝 

分 类 号：R978.5[医药卫生—药品]