基于质谱的单细胞分析技术研究新进展  

New Advances of Single-cell Analysis Based on Mass Spectrometry

作　　者：尤穆英楠 刘心昱 石先哲[1,2] 许国旺 YOU Mu-ying-nan;LIU Xin-yu;SHI Xian-zhe;XU Guo-wang(CAS Key Laboratory of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;Liaoning Key Laboratory of Metabolomics,Dalian 116023,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁大连116023 [2]辽宁省代谢组学重点实验室,辽宁大连116023 [3]中国科学院大学,北京100049

出　　处：《质谱学报》2024年第3期316-331,I0001,共17页Journal of Chinese Mass Spectrometry Society

基　　金：国家重点研发计划项目(2022YFC3401200);国家自然科学基金项目(22274156)。

摘　　要：细胞是生物体最基本的结构和功能单位。近年来,人们对细胞异质性的关注越来越多。为了分析细胞的这种异质性,避免单细胞信息被群体细胞的平均值所淹没,单细胞分析技术不断发展起来。由于单细胞的体积小,内含物质含量低且种类繁多,某些物质在细胞内的变化迅速,分析过程中不同物质之间相互干扰,造成了单细胞分析极具挑战性。基于质谱的单细胞分析技术具有通用性强、无需标记、高灵敏度、高分辨率、高选择性的优点,已逐渐在单细胞分析技术中脱颖而出,成为单细胞分析的理想工具。本文系统地总结了近5年来基于质谱的单细胞分析技术的最新进展,包括电喷雾电离质谱、激光解吸电离质谱、二次离子质谱、电感耦合等离子体质谱,并对未来的研究和技术方向进行展望,希望为单细胞质谱分析技术的研究提供参考。Cells are the most basic structural and functional unit of living organisms.In recent years,people have paid more attention to cell heterogeneity.In order to characterize the heterogeneity of cells and prevent the life information in an individual cell from being overwhelmed by the average level of the population gained from traditional cell biology research,many new analytical techniques have been developed for single-cell analysis.However,single-cell analysis is extremely challenging due to the minimal cell volume,low content and wide variety of analytes in single cells,some of analytes change rapidly in cells and others may interfere with each other during analysis.Mass spectrometry has gradually become an ideal tool for single-cell analysis due to its high sensitivity,high resolution and high selectivity.In this review,several techniques of single-cell analysis based on mass spectrometry in the recent five years were systematically summarized and discussed including electrospray ionization mass spectrometry(ESI-MS),laser desorption ionization mass spectrometry(LDI-MS),secondary ion mass spectrometry(SIMS)and inductively coupled plasma mass spectrometry(ICP-MS).Finally,this review also looked into future developments of these mass spectrometry-based techniques.With the development of cell biology,the requirement for single-cell analysis technology is increasing as well.In the meanwhile,single-cell analysis,which focuses on studying the mechanisms of cellular and molecular behavior,has aroused more and more attention from various branches of life sciences in recent years.Although higher spatial resolution and detection sensitivity have become the development trends for single-cell mass spectrometry,none of these mass spectrometry-based techniques can be recognized flawless,which means they all have certain flaws to some extent.Consequently,single-cell analysis technology based on mass spectrometry is developing towards methodological diversification.On the one hand,it is of great importance to choose one suitab

关 键 词：单细胞 电喷雾电离质谱(ESI-MS) 激光解吸电离质谱(LDI-MS) 二次离子质谱(SIMS) 电感耦合等离子体质谱(ICP-MS) 

分 类 号：O657.63[理学—分析化学]