棘皮动物弧菌诱导中间球海胆免疫致敏现象分析  

Analysis of immune priming phenomenon in sea urchin(Strongylocentrotus intermedius)induced by Vibrio echinoideorum

作　　者：殷维骏 刘岩松 田文卓 欧凡江 刘雷 丁君[1] 张伟杰[1] 常亚青[1] YIN Weijun;LIU Yansong;TIAN Wenzhuo;OU Fanjiang;LIU Lei;DING Jun;ZHANG Weijie;CHANG Yaqing(Key Laboratory of Mariculture&Stock Enhancement in North China s Sea,Ministry of Agriculture and Rural Affairs,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]大连海洋大学农业农村部北方海水增养殖重点实验室,辽宁大连116023

出　　处：《大连海洋大学学报》2024年第2期266-274,共9页Journal of Dalian Ocean University

基　　金：辽宁省教育厅基本科研面上项目(LJKZ0702);辽宁省海洋经济发展项目(20220001);2023中央财政对辽宁渔业补助项目;大连市重点研发计划项目(2022YF16SN067);农业农村部农业科研杰出人才及创新团队项目。

摘　　要：为探究中间球海胆(Strongylocentrotus intermedius)是否存在免疫致敏现象,使用棘皮动物弧菌(Vibrio echinoideorum)对中间球海胆进行重复感染(首次、二次菌浓度分别为10^(3)、10^(4) CFU/mL),检测并比较了两次注射感染后不同时间点中间球海胆体腔细胞密度、吞噬能力、酸性磷酸酶(ACP)活力、活性氧(ROS)含量、总抗氧化能力(T-AOC)等相关免疫指标的变化,以及二次感染后免疫致敏组和对照组间成活率的差异。结果表明:与首次感染相比,二次感染时诱导致敏组的体腔细胞总密度、吞噬细胞密度、ACP、ROS和T-AOC等均出现显著上升(P<0.05),且响应时间明显缩短,达到峰值的时间分别提前了36、36、84、12、36 h,且各指标的峰值均显著高于首次感染(P<0.05);二次感染后,诱导致敏组在6 h时的吞噬率(45.41%±6.39%)显著高于对照组(33.17%±1.94%);经过棘皮动物弧菌低浓度诱导后,诱导致敏组存活率(43.33%±10.00%)显著高于未经诱导的阳性对照组(7.78%±10.71%)(P<0.05)。研究表明,低浓度棘皮动物弧菌能诱导中间球海胆产生免疫致敏现象,此结果可为中间球海胆的疾病防控提供新思路。To investigate whether immune priming exists in sea urchin(Strongylocentrotus intermedius),twice infection with Vibrio echinoideorum was conducted.Differences in immune parameters such as phagocytic amoebocyte density,red spherule cell density,phagocytic rate,phagocytic index,acid phosphatase activity(ACP),reactive oxygen species(ROS)content,and total antioxidant capacity(T-AOC)between the two time infections were compared.Difference in survival rate post challenge between immune priming group and control group were also compared.The results showed that the total coelomocytes cell density,phagocyte density,ACP,ROS and T-AOC in the induced immune priming group increased significantly after the second infections(P<0.05),and the response time to the maximum was 36,36,84,12 and 36 h earlier respectively,and the peak values were significantly higher than those in the first infection(P<0.05).The phagocytosis rate was significantly higher in the induced priming group(45.41%±6.39%)than that in the control group(33.17%±1.94%)at 6 h post infection.The survival rate was significantly higher in the induced immune priming group(43.33%±10.00%)than that in the uninduced positive control group(7.78%±10.71%)(P<0.05).The findings indicate that low concentrations of V.echinoideorum can induce immune priming in the sea urchin,which provides new ideas for prevention and control of diseases in sea urchin.

关 键 词：中间球海胆 棘皮动物弧菌 免疫致敏 免疫指标 

分 类 号：S947.9[农业科学—水产养殖]