Engineering Shewanella-re duce d graphene oxide aerogel biohybrid to efficiently synthesize Au nanoparticles  

作　　者：Baocai Zhang Shulin Shao Huan Yu Qijing Liu Sicheng Shi Qingyuan Wu Zijie Wu Yiyu Feng Yifei Wang Xi Sun Deguang Wu Feng Li Hao Song 

机构地区：[1]Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),Tianjin University,Tianjin 300072,China [2]Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China [3]Beijing Advanced Innovation Center for Soft Matter Science and Engineering,Beijing University of Chemical Technology,Beijing 100029,China [4]School of Materials Science and Engineering,Tianjin University,Tianjin 300350,China [5]College of Biological Engineering,Tianjin Agricultural University,Tianjin 300384,China [6]Department of Brewing Engineering,Moutai Institute,Luban Ave,Renhuai 564507,China

出　　处：《Journal of Materials Science & Technology》2024年第1期50-61,共12页材料科学技术（英文版）

基　　金：supported by the National Key Research and Development Program of China(No.2018YFA0901300);the Na-tional Natural Science Foundation of China(Nos.NSFC 32071411,NSFC 32001034,and NSFC 31701569);the Young Science and Tech-nology Talents Growth Project of Education Department of Guizhou Province(No.KY[2018]445);Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation,China National Light Indus-try(No.2021JJ013).

摘　　要：Biosynthesizing Au nanoparticles(AuNPs)from gold-bearing scraps provides a sustainable method to meet the urgent demand for AuNPs.However,it remains challenging to efficiently biosynthesize AuNPs of which the diameter is less than 10 nm from a trace amount of Au^(3+)concentration at the level of tens ppm.Here,we constructed an exoelectrogenic cell(eCell)-conductive reduced-graphene-oxide aero-gel(rGA)biohybrid by assembling Shewanella sp.S1(SS1)as living biocatalyst and rGA as conductive ad-sorbent,in which Au^(3+)at trace concentrations would be enriched by the adsorption of rGA and reduced to AuNPs through the extracellular electron transfer(EET)of SS1.To regulate the size of the synthe-sized AuNPs to 10 nm,the strain SS1 was engineered to enhance its EET,resulting in strain RS2(pYYD-P tac-ribADEHC&pHG13-P_(bad)-omcC in SS1).Strain RS2 was further assembled with rGA to construct the RS2-rGA biohybrid,which could synthesize AuNPs with the size of 7.62±2.82 nm from 60 ppm Au^(3+)so-lution.The eCell-rGA biohybrid integrated Au^(3+)adsorption and reduction,which enabled AuNPs biosyn-thesis from a trace amount of Au^(3+).Thus,the required Au^(3+)ions concentration was reduced by one or two orders of magnitude compared with conventional methods of AuNPs biosynthesis.Our work devel-oped an AuNPs size regulation technology via engineering eCell’s EET with synthetic biology methods,providing a feasible approach to synthesize AuNPs with controllable size from trace level of gold ions.

关 键 词：Au nanoparticles Exoelectrogenic cell(e-Cell) SHEWANELLA Synthesis biology Extracellular electron transfer Reduced graphene oxide aerogel 

分 类 号：O65[理学—分析化学]