正交设计优化蟾酥注射液的提取工艺  

Optimization of extraction technology of toad venom injection by orthogonal design

作　　者：刘城志 吴莉芩 彭健波 程楸琪 吴春璇 卢嘉烨 梁正敏[1,3] 何家康 Liu Chengzhi;Wu Liqin;Peng Jianbo;Cheng Qiuqi;Wu Chunxuan;Lu Jiaye;Liang Zhengmin;He Jiakang(College of Animal Science and Technology,Guangxi University,Guangxi Nanning 530003;Guangxi Vocational University of Agriculture,Guangxi Nanning 530009;Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics,Guangxi Nanning 530003)

机构地区：[1]广西大学动物科学技术学院,广西南宁530003 [2]广西农业职业技术大学,广西南宁530009 [3]广西兽用生物制品工程研究中心,广西南宁530003

出　　处：《现代畜牧兽医》2024年第2期23-27,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：大学生创新创业训练计划项目(202210593097);广西重点研发计划项目(桂科AB23026082)。

摘　　要：试验旨在优化蟾酥注射液的提取工艺,试验以吲哚类总生物碱(以5-羟色胺盐酸盐计)的含量为考察指标,以加水倍数(A)、加热温度(B)和提取时间(C)为考察因素,通过L_(9)(3^(4))正交试验优化蟾酥注射液的提取工艺,确定提取工艺参数,并采用最大极差法对正交试验数据分析处理。结果显示,加水倍数是影响蟾酥注射液主要成分含量的主要因素,蟾酥注射液的最佳提取工艺为10倍加水量浸泡,加热煮沸(100℃),提取时间4 h。验证性试验按最优工艺制备3批样品,采用紫外可见分光光度(UV)法测定蟾酥注射液中吲哚类总生物碱的含量为9.92 mg/L。研究表明,该提取工艺稳定可靠,可用于中试生产。The purpose of the experiment was to optimize the extraction process of toad venom injection.The concentrations of indole alkaloids(calculated by 5-hydroxytryptamine hydrochloride)was taken as the index,and the water addition multiple(A),heating temperature(B)and extraction time(C)were taken as the factors.The extraction process of toad venom injection was optimized by L_(9)(3^(4))orthogonal test,and the extraction process parameters were determined.The data of orthogonal test are analyzed and processed by the maximum range method.The results showed that the ratio of adding water was the main factor affecting the concentrations of main components in the toad venom injection,and the best extraction process of toad venom injection was soaking in 10 times of water,heating and boiling(100℃),and extracting time was 4 h.Three batches of samples were prepared according to the optimum technology,and the content of indole alkaloids in toad venom injection was determined to be 9.92 mg/L by the ultraviolet visible spectrophotometry(UV)method.The research shows that the extraction process is stable and reliable,and can be used in pilot production.

关 键 词：蟾酥注射液 提取工艺 正交试验 UV法 5-羟色胺盐酸盐 

分 类 号：S859.53[农业科学—临床兽医学]