人类辅助生殖技术受孕的稽留流产患者绒毛组织染色体异常结果分析  

Analysis of chromosomal abnormalities in villi tissues in patients with missed abortion conceived by human assisted reproductive technology

作　　者：杨明雪 翟俊英[1] 钮红丽[1] YANG Mingxue;ZHAI Junying;NIU Hongli(Department o Reproductive Medicine,Nanyang First People′s Hospital,Nanyang,Henan 473000,China)

机构地区：[1]南阳市第一人民医院生殖医学科,河南南阳473000

出　　处：《现代医药卫生》2024年第9期1516-1520,共5页Journal of Modern Medicine & Health

摘　　要：目的分析不同辅助生殖技术助孕的稽留流产患者绒毛组织染色体异常结果。方法选取2019年7月至2023年7月该院收治的接受辅助受孕的稽留流产患者260例作为辅助受孕组,再根据接受的不同辅助生殖技术分为体外受精(IVF)组(135例)、人工授精(AIH)组(45例)和卵胞质内单精子显微注射(ICSI)组(80例),并选取自然受孕的稽留流产患者42例作为对照组,采集各组患者绒毛组织,采用高通量测序法进行绒毛组织染色体检测,观察各组患者染色体拷贝数变异(CNV)和数目异常情况。结果辅助受孕组患者染色体异常检出率、CNV异常率、数目异常类型与对照组比较,差异均无统计学意义(P>0.05)。IVF组患者染色体异常检出率、CNV异常率、数目异常类型与AIH组、ICSI组比较,差异均无统计学意义(P>0.05)。辅助受孕组患者中染色体数目异常者三体中以16/22/21-三体异常染色体为主,双三体异常以48,XX+3,+18、48,XXY,+21为主,X单体异常均为45,X;三倍体异常患者中5例患者为69,XXY,2例患者为69,XXX。各组患者染色体异常核型分布情况比较,差异均无统计学意义(P>0.05)。结论辅助生殖技术不会增加染色体异常风险,且异常染色体核型分布情况与辅助生殖技术方式无关,可根据患者的整体情况进行辅助受孕方式的选择。Objective To analyze the chromosomal abnormalities in villus tissue of missed abortion patients with different assisted reproductive techniques.Methods A total of 260 missed abortion patients admitted to the hospital from July 2019 to July 2023 who received assisted conception were selected as the assisted conception group.According to the different assisted reproductive techniques,they were divided into the in-vitro fertilization(IVF)group(135 cases),the artificial fertilization(AIH)group(45 cases)and the intracytoplasmic sperm injection(ICSI)group(80 cases).And a total of 42 missed abortion patients with natural conception were selected as the control group,and the chorionic tissue of the patients in each group was collected.The chromosome copy number variation(CNV)and abnormal number of chromosomes were observed by high-throughput sequencing.Results There was no significant difference in chromosome abnormality detection rate,CNV abnormality rate and number abnormality type between the assisted conception group and the control group(P>0.05).There was no significant difference in chromosome abnormality detection rate,CNV abnormality rate and number abnormality type in the IVF group compared with the AIH group and the ICSI group(P>0.05).In the assisted conception group,16/22/21-trisomy abnormalities were predominant in the patients with abnormal chromosome numbers,while ditrisomy abnormalities were predominant in 48,XX+3,+18,48,XXY,+21,and X monomer abnormalities were all 45,X.Among the patients with triploid abnormalities,five patients were 69,XXY and two patients were 69,XXX.There was no significant difference in the distribution of abnormal karyotypes among all the groups(P>0.05).Conclusion Assisted reproductive technology does not increase the risk of chromosomal abnormalities,and the distribution of abnormal chromosome karyotypes has nothing to do with the assisted reproductive technology method,and the assisted conception method can be selected according to the overall situation of the patient.

关 键 词：辅助生殖技术 稽留流产 绒毛组织 染色体 

分 类 号：R714.21[医药卫生—妇产科学]