直接PCR与多重实时PCR检测临床标本的实用性评估  

Evaluation of the practicability of direct PCR versus multiple real-time PCR for testing clinical specimens

作　　者：臧晓慧 梁官钊 刘维达[1] ZANG Xiaohui;LIANG Guanzhao;LIU Weida(Hospital for Skin Diseases,Institute of Dermatology,Chinese Academy of Medical Sciences&Peking Union Medical College,Nanjing 210042,China)

机构地区：[1]北京协和医学院/中国医学科学院/皮肤病医院/皮肤病研究所,南京210042

出　　处：《中国真菌学杂志》2024年第1期43-46,共4页Chinese Journal of Mycology

摘　　要：目的评估与传统方法相比,直接PCR与DG(Derma Genius)多重实时PCR在鉴定皮肤癣菌菌种的临床实用性。方法收集2020年3月—2021年3月中国医学科学院皮肤病医院184例由临床医师怀疑浅部真菌感染的皮屑或甲屑标本,分别行显微镜检、培养、直接PCR和DG多重实时PCR。以镜检和培养为金标准,比较两种PCR方法鉴定皮肤癣菌的有效性。结果直接PCR和DG-PCR的灵敏度分别为64%、99.2%,特异度分别为99.6%、88.1%,阳性预测值分别为97.5%、94.7%,阴性预测值分别为55.9%、98.1%。125例阳性标本中,培养出71例(56.8%),直接PCR检测出81例(64.8%),DG-PCR检测出99例(79.2%)。结论DG-PCR、直接PCR在检测皮肤癣菌感染方面有较高的实用价值。Objective To evaluate the clinical applicability of direct PCR and DG multiplex real-time PCR in the identification of dermatophytes strains compared with traditional methods.Methods A total of 184 samples of skin or nail scraps suspected of dermatophytosis were collected and divided into 4 samples.They were examined by direct microscopy,cultured,direct PCR and DG multiple real-time PCR,respectively.Using microscopic examination and culture as the gold standard,the efficacy of two skin PCR methods for identifying dermatomycosis was compared.Results The sensitivity of direct PCR and DG-PCR were 64%and 99.2%,respectively.The specificity was 99.6%and 88.1%,respectively.The positive predictive values were 97.5%and 94.7%,respectively.The negative predictive values were 55.9%and 98.1%,respectively.Among the 125 positive samples,71 cases(56.8%)were detected by culture,81 cases(64.8%)by direct PCR,and 99 cases(79.2%)by DG-PCR.Conclusion DG-PCR and direct PCR have high practical value in detecting dermatophyton infection.

关 键 词：皮肤癣菌病 PCR DG-PCR 

分 类 号：R446.5[医药卫生—诊断学]