基于细胞膜色谱技术的中药复杂体系目标物筛选分析装备  

Equipment for screening and analysis of target substances in the complex system of traditional Chinese medicine based on cell membrane chromatography technology

作　　者：贺浪冲[1] 贺怀贞[1] 韩省力[1] 张涛[1] 王程 马维娜[1] HE Langchong;HE Huaizhen;HAN Shengli;ZHANG Tao;WANG Cheng;MA Weina(School of Pharmacy,Xi'an Jiaotong University,Xi’an,710061,China)

机构地区：[1]西安交通大学药学院,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2024年第3期351-359,共9页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金重点项目(No.81230079,No.81930096);国家自然科学基金重大科研仪器研制项目(No.81227802);陕西省重点研发计划新冠肺炎疫情防控科研攻关应急专项(No.2022ZDXM-SF-03)。

摘　　要：中药是临床防治疾病的有效药物。由于中药物质基础的复杂性以及成药过程的多环节因素,实现中药全面质量控制的难度相对较大。如果能够在明确中药有效物质与有害物质的基础上,在成药过程中重点控制其含量和限量,则能在很大程度上“纲举目张”,有效保障中药质量的可控性和重复性。当前,我国药物研发源头创新技术不足,研发效率有待提升,缺乏高通量的精准分析装备。我国医药分析装备长期依赖进口,高端智能分析装备缺乏,亟需构建“医药智能分析系统”。本项目在国家自然科学基金重大仪器研制项目的资助下,利用细胞膜色谱技术的原创性与独创性,结合生物工程技术和人工智能技术,研制二维细胞膜色谱(2D/CMC)分析仪,对成型的“2D/CMC-中药药效物质分析仪”和“2D/CMC-中药注射液类过敏物分析仪”开展了示范应用,提升了中药药效物质和过敏组分的筛选、发现的效率,并同步进行定性、定量分析,为中药提质增效提供高效分析工具。Traditional Chinese medicine(TCM)is extensively utilized for clinical disease prevention and treatment.However,due to the intricate nature of its material basis and the multiple factors involved in the preparation process,ensuring comprehensive quality control of TCM proves to be challenging.By instilling a clear understanding of its effective and harmful substances and implementing control over the content and limit of TCM during the preparation process,the controllability and repeatability of its quality can be guaranteed.Currently,China is facing a dearth of innovative technology for drug development,necessitating an increase in research and development efficiency,especially in the realm of high-throughput precision analytical equipment.The country has long relied on imported pharmaceutical analysis equipment with a particular efficiency in high-end intelligent analysis equipment.This is especially concerning considering the urgent requirement to establish a“pharmaceutical intelligent analysis system.”This project,supported by the Major Instrument Development Project of the National Science and Technology Funds,employs cell membrane chromatography technology,complemented by biotechnology and artificial intelligence technology,to devise a two-dimensional cell membrane chromatography(2D/CMC)analyzer.The project has successfully conducted a demonstration application of the“2D/CMC-Traditional Chinese Medicine Pharmacodynamic Substance Analyzer”and the“2D/CMC-Traditional Chinese Medicine Injection Allergen Analyzer”.These tools have enhanced the screening and discovery efficiency of TCM's effective substances and allergen components.Moreover,the equipment amalgamates qualitative and quantitative analysis,thereby serving as an effective analytical tool to enhance the quality and efficacy of traditional Chinese medicine.

关 键 词：细胞膜色谱 中药 筛选 分析装备 

分 类 号：R917[医药卫生—药物分析学]