漆黄素抗大鼠静脉血栓形成的作用及机制  

Effect of fisetin against venous thrombosis in rats and its mechanism

作　　者：龙丽辉[1] 魏双 刘青 姚杨[1] 董娟妮[1] 常媛媛 文恩辉 LONG Lihui;WEI Shuang;LIU Qing;YAO Yang;DONG Juanni;CHANG Yuanyuan;EN Enhui(Department of Clinical Pharmacy,the First Affiliated Hospital of Xi'an Medical College,Xi’an 710077,China)

机构地区：[1]西安医学院第一附属医院临床药学室,陕西西安710077

出　　处：《西安交通大学学报（医学版）》2024年第3期383-387,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科技计划项目(No.2017SF-345);西安医学院第一附属医院科研项目(No.XYFYPT-2020-02)。

摘　　要：目的分析漆黄素对大鼠静脉血栓形成的影响。方法70只SD大鼠随机分为假手术组、模型组及45、15、5 mg/kg漆黄素组和阿司匹林组(47 mg/kg),分别每天1次灌胃相应药物(假手术组和模型组分别给予5 g/L羧甲基纤维素钠溶液10 mL/kg),连续7 d。末次给药1 h后,麻醉大鼠,用丝线结扎下腔静脉和左肾静脉交叉下方部位(假手术组不结扎),缝合腹壁。2 h后重新打开腹腔,用动脉夹封闭距结扎处1.5 cm外的其他静脉分支,腹主动脉采血(以38 g/L枸橼酸钠∶全血为1∶9抗凝);采血后剪取下腔静脉和左肾静脉交叉结扎点远心端1 cm静脉血栓,分离血栓,用滤纸吸干残血,称重并记录。抗凝血离心后取血浆,按ELISA试剂盒检测血浆抗凝血酶Ⅲ(AT-Ⅲ)、蛋白酶C(PC)、纤溶酶原(PLG)和纤溶酶原激活物抑制剂(PAI-1)的含量。结果与模型组相比,45 mg/kg漆黄素组和阿司匹林47 mg/kg组血栓质量降低(P<0.01);漆黄素3个剂量组AT-Ⅲ含量升高(均P<0.05);45 mg/kg漆黄素组PC含量升高,而PLG和PAI-1含量均降低(均P<0.05)。结论漆黄素具有体内抗静脉血栓形成的作用,其作用与AT-Ⅲ和PC上调,PLG和PAI-1下调有关。Objective To analyze the effect of fisetin against venous thrombosis in rats.Methods Seventy SD rats were randomly divided into the following groups:sham-operation group,model group,fisetin 45 mg/kg,15 mg/kg,5 mg/kg groups,and aspirin group(47 mg/kg).The corresponding medication was administered by gavage once a day consecutively(the sham-operation group and the model group were given 0.5%carboxymethyl cellulose sodium solution with 10 mL/kg,respectively)for 7 consecutive days.One hour after the last administration,the rats were anesthetized,the lower part of the intersection of inferior vena cava and left renal vein was ligated with silk thread(no ligation in the sham-operation group),and the abdominal wall was sutured.Two hours later,the abdominal cavity was reopened,the other venous branches 1.5 cm away from the ligation site were closed with the artery clamp,and blood was collected from the abdominal aorta.The anticoagulant ratio of 3.8%sodium citrate∶whole blood was 1∶9.The venous thrombus 1 cm down from the ligation point of the intersection of inferior vena cava and left renal vein was cut and the thrombus was separated.The residual blood was dried with filter paper,weighed and recorded.Plasma was taken after anticoagulant blood centrifugation.The levels of plasma antithrombin-Ⅲ(AT-Ⅲ),protease C(PC),plasminogen(PLG),and plasminogen activator inhibitor(PAI-1)were detected by ELISA kits.Results Compared with the model group,the weight of thrombus in fisetin 45 mg/kg group and aspirin 47 mg/kg group decreased(P<0.01).The content of AT-Ⅲin three fisetin groups increased(all P<0.05).The content of PC in fisetin 45 mg/kg increased(P<0.05).The content of PLG and PAI-1 in fisetin 45 mg/kg group decreased(both P<0.05).Conclusion Fisetin has the effect against venous thrombosis in vivo,and the effect is related to the upregulation of AT-Ⅲand PC and the downregulation of PLG and PAI-1.

关 键 词：漆黄素 大鼠 静脉血栓 凝血因子 

分 类 号：R965.1[医药卫生—药理学]