microRNA基因多态性与血压钠钾反应性的相关性分析  

Association of gene polymorphisms in microRNA with blood pressure responses to salt and potassium intake

作　　者：王兰 崔莹 郭艳杰 姚艳妮 杨贝贝 刘乃溶 王佳馨 刘盼盼 杜鸣飞 胡桂霖 牛泽家馨 张玺 王丹[3] 褚超[3] 贾昊 孙月 高卫华[4] 牟建军[3] 汪洋[3] WANG Lan;CUI Ying;GUO Yanjie;YAO Yanni;YANG Beibei;LIU Nairong;WANG Jiaxin;LIU Panpan;DU Mingfei;HU Guilin;NIU Zejiaxin;ZHANG Xi;WANG Dan;CHU Chao;JIA Hao;SUN Yue;GAO Weihua;MU Jianjun;WANG Yang(Department of Cardiology,Xi'an International Medical Center Hospital,Xi’an 710100;Department of Neurorehabilitation,Xi'an International Medical Center Hospital,Xi’an 710100;Department of Cardiovascular Medicine,The First Affiliated Hospital of Xi'an Jiaotong University,Xi’an 710061;Department of Cardiology,Xi'an No.1 Hospital,Xi’an 710002,China)

机构地区：[1]西安国际医学中心医院心内科,陕西西安710100 [2]西安国际医学中心医院神经康复三科,陕西西安710100 [3]西安交通大学第一附属医院心内科,陕西西安710061 [4]西安市第一医院心内科,陕西西安710002

出　　处：《西安交通大学学报（医学版）》2024年第3期435-442,共8页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.82070437);陕西省自然科学基金项目(No.2021JM-257,2021JM-588);西安交通大学第一附属医院临床研究项目(No.XJTU1AF-CRF-2022-002,XJTU1AF2021CRF-021)。

摘　　要：目的探讨miRNA基因多态性与钠钾饮食干预后血压反应性之间的关系。方法本课题组2004年从中国陕西宝鸡7个村庄的124个家庭中招募514名参与者进行慢性盐负荷试验干预,包括3 d基线期、7 d低盐饮食、7 d高盐饮食和7 d高盐补钾饮食干预。纳入19个miRNA-SNP位点进行分析研究。结果在钠钾饮食干预过程中,受试者的血压在低盐期呈下降趋势,高盐期呈现上升趋势,而在高盐补钾后,血压则再次下降。在低盐期,miR-210-3p SNP rs12364149与收缩压反应性、舒张压反应性及平均动脉压反应性显著相关,miR-4638-3p SNP rs6601178与收缩压反应性显著相关,而miR-26b-3p SNP rs115254818与平均动脉压反应性显著相关。在高盐干预后,miR-26b-3p SNP rs115254818与收缩压反应性、舒张压反应性及平均动脉压反应性显著相关;miR-1307-5p SNP rs11191676、rs2292807与收缩压反应性及平均动脉压反应性密切相关;miR-4638-3p SNP rs6601178、miR-210-3p SNP rs12364149以及miR-382-5p SNP rs4906032、rs4143957与收缩压反应性存在显著关联性。此外,在给予补钾干预后miR-26b-3p SNP rs115254818与收缩压反应性、舒张压反应性及平均动脉压反应性关联显著;miR-1307-5p SNP rs11191676、rs2292807以及miR-19a-3p SNP rs4284505与收缩压反应性显著相关。结论miRNA基因多态性与血压钠钾反应性密切相关,提示miRNA基因可能参与血压盐敏感性及钾敏感性的形成。Objective To investigate the relationship of miRNA gene polymorphisms with blood pressure(BP)responses to the sodium and potassium diet intervention.Methods In 2004,we recruited 514 participants from 124 families in seven villages of Baoji,Shaanxi Province,China.All subjects were given a three-day normal diet,followed by a seven-day low-salt diet,a seven-day high-salt diet,and finally a seven-day high-salt and potassium supplementation.A total of 19 miRNA single nucleotide polymorphisms(SNPs)were selected for analysis.Results Throughout the sodium-potassium dietary intervention,the BP of the subjects fluctuated across all phases,showing a decrease during the low-salt period and an increase during the high-salt period,followed by a reduction in BP subsequent to potassium supplementation during the high-salt diet.MiR-210-3p SNP rs12364149 was significantly associated with systolic BP(SBP),diastolic BP(DBP)and mean arterial pressure(MAP)responses to low-salt diet.MiR-4638-3p SNP rs6601178 was significantly associated with SBP while miR-26b-3p SNP rs115254818 was significantly associated with MAP responses to low-salt intervention.In addition,miR-26b-3p SNP rs115254818 was significantly correlated with SBP,DBP and MAP responses to high-salt intervention.MiR-1307-5p SNPs rs11191676 and rs2292807 were associated with SBP and MAP responses to high-salt diet.MiR-4638-3p SNP rs6601178,miR-210-3p SNP rs12364149,miR-382-5p SNP rs4906032 and rs4143957 were significantly associated with SBP response to high-salt diet.In addition,miR-26b-3p SNP rs115254818 was significantly associated with SBP,DBP and MAP responses to potassium supplementation.MiR-1307-5p SNPs rs11191676,rs2292807,and miR-19a-3p SNP rs4284505 were significantly associated with SBP responses to high-salt and potassium supplementation.ConclusionmiRNA gene polymorphisms are associated with BP response to sodium and potassium,suggesting that miRNA genes may be involved in the pathophysiological process of salt sensitivity and potassium sensitivity.

关 键 词：miRNA 基因多态性 盐敏感性 血压的钠钾反应性 

分 类 号：R3[医药卫生—基础医学] R544