孕期应激致卵巢恢复功能减弱及氧化应激水平增加  

Weakened ovarian recovery function and increased oxidative stress level due to prenatal stress

作　　者：吴文星 高力健 李晓晓[2] 白云飞[3] 徐志卿[2,3] 石翠格 李慧[1] WU Wen-xing;GAO Li-jian;LI Xiao-xiao;BAI Yun-fei;XU Zhi-qing;SHI Cui-ge;LI Hui(Department of Human Anatomy,School of Basic Medicine Sciences,Capital Medical University,Beijing 100069;Department of Neurobiology,School of Basic Medical Sciences,Capital Medical University,Beijing 100069;Department of Pathology,School of Basic Medical Sciences,Capital Medical University,Beijing 100069;National Research Institute of Family Planning,Beijing 100081)

机构地区：[1]首都医科大学基础医学院人体解剖学教研室,北京100069 [2]首都医科大学基础医学院神经生物学系,北京100069 [3]首都医科大学基础医学院病理学系,北京100069 [4]国家卫生健康委科学技术研究所,北京100081

出　　处：《生殖医学杂志》2024年第5期612-617,共6页Journal of Reproductive Medicine

基　　金：国家自然科学基金面上项目(82071514,82271551,82174116);北京市教委一般项目(KM202110025029)。

摘　　要：目的利用孕期束缚应激诱导的产后抑郁大鼠模型,探索孕期应激对卵巢生殖激素分泌水平及氧化应激反应等的影响。方法孕鼠随机分为两组,每组6只。对照组不做处理,实验组(PPD组)在孕8~21 d进行为期两周、每天2 h的束缚应激,产后1周内进行抑郁行为的检测。分娩后采用酶联免疫吸附法检测血浆卵泡刺激素(FSH)和黄体生成素(LH)的水平;蛋白免疫印迹方法检测卵巢ERα、ERβ、ERK1/2、Nrf2、HO-1以及炎症因子IL-6的表达水平;HE染色观察卵泡发育情况。结果孕期束缚后,与对照组大鼠比较,PPD组的糖水偏好率显著降低(P<0.01)且强迫游泳不动时间显著延长(P<0.001),血浆FSH水平显著降低(P<0.001)、LH水平显著升高(P<0.01)。与对照组大鼠比较,PPD组的卵巢组织中ERβ、p-ERK1/2、Nrf2、HO-1蛋白相对表达量显著降低(P<0.05),而炎症因子IL-6蛋白相对表达量显著升高(P<0.05)。HE染色结果显示,PPD组大鼠卵巢组织中闭锁卵泡增多。结论孕期束缚应激可导致大鼠卵巢组织中氧化应激及炎性因子水平增加,闭锁卵泡增多,提示孕期束缚应激可致产后卵巢的恢复能力减弱。Objective:A rat model of postpartum depression(PPD)induced by restraint stress during pregnancy was used to explore the effects of prenatal stress on ovarian reproductive hormone secretion and oxidative stress response.Methods:Rats were divided into two groups(6 rats per group).Those in the control group received no intervention and those subjected to restraint stress every 2 hours from 8 to 21 days of gestation were as the PPD group.The depressive behavior was detected within one week after delivery.The plasma levels of FSH and LH were detected by ELISA.The expression of ERα,ERβ,Nrf2,HO-1,ERK1/2,p-ERK1/2,IL-6 were detected by Western blot.HE staining was used to observe the development of follicles.Results:Compared with the control group,the preference rate of sugar and water in PPD group was significantly decreased(P<0.01),and the immobility time of forced swimming was significantly prolonged(P<0.001).Plasma FSH level decreased(P<0.001),while LH level increased(P<0.01).The protein levels of ERβ,p-ERK1/2,Nrf2 and HO-1 in ovary were decreased,but the protein level of IL-6 was increased(P<0.05).HE staining showed that the number of atretic follicle of rat ovary in PPD group was increased.Conclusions:Restraint stress during pregnancy can increase oxidative stress response and inflammatory factors in ovary,suggesting that restraint stress during pregnancy can reduce the recovery ability of the ovary after delivery.

关 键 词：产后抑郁 卵巢 氧化应激 炎性因子 

分 类 号：Q492.6[生物学—生理学]