一株红树林来源放线菌的抗菌活性物质  

Antimicrobial compounds of an actinomycete derived from mangrove sediments

作　　者：张骏梁 史蔷 吴昊 张萌 徐颖 ZHANG Junliang;SHI Qiang;WU Hao;ZHANG Meng;XU Ying(College of Life Sciences and Oceanography,Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science,Shenzhen Engineering Laboratory for Marine Algal Biotechnology,Shenzhen University,Shenzhen 518071,Guangdong Province,P.R.China)

机构地区：[1]深圳大学生命与海洋科学学院,深圳市海洋生物资源与生态环境重点实验室,深圳市海洋藻类生物开发与应用工程实验室,广东深圳518071

出　　处：《深圳大学学报（理工版）》2024年第3期358-366,共9页Journal of Shenzhen University(Science and Engineering)

基　　金：国家重点研发计划资助项目(2019YFA0902500);深圳市基础研究计划资助项目(JCYJ20210324093409025)。

摘　　要：红树林沉积物微生物资源丰富,是新型天然产物以及药物先导化合物的重要来源之一.为获得具有新颖结构的微生物次级代谢产物,以活性为导向对红树林来源菌株进行筛选,对表现出抗细菌、生物膜和真菌活性的GP3T5(Streptomyces sp.)菌株的次级代谢产物进行研究.通过萃取、C18反相硅胶柱层析、Sephadex LH20凝胶柱层析和制备高效液相色谱,从菌株发酵液中分离出3种化合物,分别记为化合物1、2和3.利用高分辨电喷雾质谱、核磁共振波谱和紫外光谱等技术,鉴定化合物1、2和3依次为pyrisulfoxin A、collismycin A和echinomycin A.对化合物1、2和3进行活性评价,结果表明,3种化合物均有不同程度的抗菌和抗生物膜活性.其中,化合物3的抗菌活性最好,对食源性病原菌有很好的抗菌活性,对单增李斯特菌和无乳链球菌的最小抑菌质量浓度均为0.06μg/mL.化合物1、2和3对鲍曼不动杆菌的最小生物膜抑制质量浓度分别为32、16和1μg/mL.化合物2和3具有良好的抗植物病原真菌活性,化合物2的半数抑制质量浓度IC50≤10μg/mL,化合物3的IC50≤1μg/mL.以上红树林来源菌株GP3T5的研究结果可为深入挖掘红树林土壤微生物生物活性物质提供参考.Mangrove-derived microorganism is a promising and important resource of novel natural products and lead compounds for drug discovery.This study aimed to find microbial secondary metabolites with novel structures.Based on the bioactive-guided screening of mangrove-derived actinomycetes,Streptomyces sp.GP3T5 was studied owing to its antibacterial,antibiofilm and anti-fungi activities.Three compounds(1-3)were isolated from the fermentation broth by organic solvent extraction,reversed-phase chromatography,Sephadex LH20 gel chromatography,and preparative high-performance liquid chromatography.Their structures were identified as pyrisulfoxin A(compound 1),collismycin A(compound 2),and echinomycin A(compound 3),respectively,via high resolution electrospray ionization mass spectroscopy(HRESIMS),nuclear magnetic resonance(NMR),ultraviolet(UV)spectroscopy.The activity evaluation of compounds 1-3 revealed that all three compounds showed antibacterial and antibiofilm activities.Compound 3 showed potent antibacterial activity against foodborne pathogens with minimum inhibitory concentrations(ρMIC)of 0.06μg/mL for Listeria monocytogenes and Streptococcus agalactiae.The minimum biofilm inhibitory concentrations(ρMBIC)of compounds 1-3 against Acinetobacter baumannii were 32μg/mL,16μg/mL,and 1μg/mL,respectively.Compounds 2 and 3 showed good antifungal activity against plant pathogenic fungi,with half maximal inhibitory concentrations(IC50)≤10μg/mL and≤1μg/mL,respectively.In conclusion,these results on strain GP3T5 derived from mangroves provide valuable insights for further exploration of bioactive compounds from mangrove soil microorganisms.

关 键 词：天然产物 红树林放线菌 链霉菌 次级代谢产物 抗菌 抗生物膜 抗食源性细菌 抗植物致病真菌 

分 类 号：Q939.9[生物学—微生物学]