microRNA-144/451在不同类型贫血性疾病中的表达  

Expression of MiR-144/451 in Different Types of Anemia

作　　者：杨帆 凌玲 侯亚颖 杨兰 孙梅[2] 王方方[2] 郁多男 YANG Fan;LING Ling;HOU Ya-Ying;YANG Lan;SUN Mei;WANG Fang-Fang;YU Duo-Nan(Yangzhou University School of Medicine,Jiangsu Key Laboratory of Experimental&Translational Non-coding RNA Research,Yangzhou 225009,Jiangsu Province,China;Clinical Medical College,Yangzhou University,Northern Jiangsu People's Hospital,Yangzhou 225001,Jiangsu Province,China)

机构地区：[1]扬州大学医学院,江苏省非编码RNA基础与临床转化重点实验室,江苏扬州225009 [2]扬州大学临床医学院,江苏省苏北人民医院,江苏扬州225001

出　　处：《中国实验血液学杂志》2024年第3期825-830,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金面上项目(81470277,81870096);江苏省(扬州大学)研究生科研与实践创新计划项目(KYCX21_3294)。

摘　　要：目的:研究micro RNA-144/451基因簇(miR-144/451)在不同类型贫血性疾病中的表达及临床相关性。方法:收集再生障碍性贫血(AA)、骨髓增生异常综合征(MDS)和弥漫大B细胞淋巴瘤(DLBCL)初诊贫血和化疗后贫血患者外周血,RT-q PCR法检测miR-144和miR-451的表达水平,采用Spearman相关性分析研究miR-144和miR-451表达与常规实验室指标的相关性。结果:与正常对照组相比,miR-144在AA和MDS患者外周血中表达均明显降低(均P<0.01),在DLBCL初诊不贫血、初诊贫血和化疗后贫血患者外周血中表达均无明显差异(P>0.05);miR-451在AA和MDS患者外周血中表达均明显降低(均P<0.01),在DLBCL各组患者外周血中表达明显升高(均P<0.05)。相关性分析结果显示,miR-144和miR-451的表达水平与AA患者红细胞分布宽度变异系数呈正相关性(r=0.629,r=0.574),与MDS和DLBCL组实验室检测指标均无明显相关性。结论:miR-144和miR-451在不同类型的贫血性疾病中表达水平不尽相同,有望成为临床贫血性疾病的辅助诊断以及鉴别诊断指标。Objective:To investigate the expression level and clinical correlation of microRNA-144/451 gene cluster(miR-144/451)in different types of anemia.Methods:The peripheral blood of patients with aplastic anemia(AA),myelodysplastic syndrome(MDS)and diffuse large B-cell lymphoma(DLBCL)who had been diagnosed with anemia for the first time and after chemotherapy were collected.The expression levels of miR-144 and miR-451 were measured by RT-qPCR,and the correlation between the expression levels of miR-144 and miR-451 and routine laboratory indexes was analyzed by Spearman correlation analysis.Results:The expression levels of miR-144 and miR-451 in the peripheral blood of AA and MDS patients were significantly lower than those in normal controls(all P<0.01).No statistical differences were observed in the expression level of miR-144 in three subgroups of DLBCL patients(P>0.05),while the expression level of miR-451 in peripheral blood of three subgroups of DLBCL patients were significantly higher than those in normal controls(all P<0.05).Correlation analysis showed that the expression levels of miR-144 and miR-451 in AA patients were positively correlated with red blood cell distribution width-coefficient of variation(RDW-CV)(r=0.629,0.574).There were no significant correlations between the expression levels of miR-144 and miR-451 and laboratory parameters in MDS and DLBCL patients.Conclusion:Different types of anemia disorders have varying levels of miR-144 and miR-451 expression,which is anticipated to develop into a secondary diagnostic and differential diagnostic indicator for clinical anemia diseases.

关 键 词：miR-144 miR-451 贫血 相关性分析 

分 类 号：R556[医药卫生—血液循环系统疾病] R733[医药卫生—内科学]