皮肤肿瘤早期筛查的快速无创电阻抗检测方法  

A Rapid Non-invasive Method for Skin Tumor Tissue Early Detection Based on Bioimpedance Spectroscopy

作　　者：彭俊文 胡松佩 洪志扬 王莉莉 刘凯[1] 姚佳烽 PENG Jun-Wen;HU Song-Pei;HONG Zhi-Yang;WANG Li-Li;LIU Kai;YAO Jia-Feng(College of Electrical and Mechanical Engineering,Nanjing University of Aeronautics and Astronautics,Nanjing 210016,China)

机构地区：[1]南京航空航天大学机电学院,南京210016

出　　处：《生物化学与生物物理进展》2024年第5期1161-1173,共13页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(62071224);江苏省自然科学基金(BK20221480)资助项目。

摘　　要：目的随着环境问题和臭氧层空洞化的加剧,皮肤肿瘤的患病率也大幅增加,但是皮肤肿瘤前期隐蔽性高、症状不明显,导致大部分病例都是在中晚期发现的。因此,本文基于生物阻抗谱(bioimpedance spectroscopy,BIS)技术,提出一种皮肤肿瘤早期筛查的快速无创电阻抗检测方法。方法首先,建立四层皮肤模型,采用数值分析方法研究角质层对BIS测量的阻碍作用。其次,使用去除角质层的皮肤模型研究混有不同半径和浸润深度的皮肤肿瘤组织电学特性。最后,使用凝胶处理后的猪皮组织实验验证肿瘤浸润深度(h)的影响。结果角质层仿真结果表明,去除角质层的皮肤对激励信号的响应更明显。皮肤肿瘤模型仿真表明,当肿瘤半径(R_(tumor))及h>1.5 mm时能够区分肿瘤组织与正常组织。同时根据实验结果中正常组织与肿瘤组织虚部弛豫阻抗(Z_(imag-relax))定义了组织病变度(ε_(worse),为肿瘤组织虚部阻抗相对于正常组织虚部阻抗变化的百分比),并绘制了肿瘤组织浸润深度(Depth)与Z_(imag-relax)的拟合曲线。当ε_(worse)=0.4920时,BIS即可识别微浸润肿瘤组织,且拟合曲线修正决定系数为0.9468,拟合效果良好。结论基于BIS技术的方法具有实时、便捷、无创的优点,有望应用于皮肤肿瘤的检测。Objective In recent years,with the intensification of environmental issues and the depletion of ozone layer,incidence of skin tumors has also significantly increased,becoming one of the major threats to people’s lives and health.However,due to factors such as high concealment in the early stage of skin tumors,unclear symptoms,and large human skin area,most cases are detected in the middle to late stage.Early detection plays a crucial role in postoperative survival of skin tumors,which can significantly improve the treatment and survival rates of patients.We proposed a rapid non-invasive electrical impedance detection method for early screening of skin tumors based on bioimpedance spectroscopy(BIS)technology.Methods Firstly,we have established a complete skin stratification model,including stratum corneum,epidermis,dermis,and subcutaneous tissue.And the numerical analysis method was used to investigate the effect of dehydrated and dry skin stratum corneum on contact impedance in BIS measurement.Secondly,differentiation effect of different diameter skin tumor tissues was studied using a skin model after removing the stratum corneum.Then,in order to demonstrate that BIS technology can be used for detecting the microinvasion stage of skin tumors,we conducted a simulation study on the differentiation effect of skin tumors under different infiltration depths.Finally,in order to verify that the designed BIS detection system can distinguish between tumor microinvasion periods,we conducted tumor invasion experiments using hydrogel treated pig skin tissue.Results The simulation results show that a dry and high impedance stratum corneum will bring about huge contact impedance,which will lead to larger measurement errors and affect the accuracy of measurement results.We extracted the core evaluation parameter of relaxed imaginary impedance(Z_(imag-relax))from the simulation results of the skin tumor model.When the tumor radius(R_(tumor))and invasion depth(h)>1.5 mm,the designed BIS detection system can distinguish between

关 键 词：皮肤癌检测 无创/实时 肿瘤早筛应用 生物阻抗谱 

分 类 号：TH772[机械工程—仪器科学与技术]