流式细胞技术在抗生素抑菌实验中的应用  

Application of Flow Cytometry in Antibiotic Antibacterial Experiments

作　　者：蒋海霞 许杰[1] JIANG Haixia;XU Jie(School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学生命科学技术学院,上海200240

出　　处：《实验室研究与探索》2024年第4期14-17,共4页Research and Exploration In Laboratory

基　　金：上海交通大学决策咨询课题项目(JCZXSJB2022-13);上海市农业科技创新项目(2023-02-08-00-12-F04596)。

摘　　要：针对抗生素类药物抑菌实验检测中存在的缺少量化指标、无法准确评估细菌自身生长状态以及实验周期较长等问题,探究不同质量浓度(0.02~20 mg/mL)和不同处理时间(1和2 h)下申嗪霉素对黄单胞菌的抑菌检测中流式细胞技术的应用。结果表明,相同处理时间下,申嗪霉素质量浓度越高,凋亡晚期的坏死细胞越多;相同质量浓度下,随着申嗪霉素处理时间的增加,死亡细胞的比例明显增加。流式细胞技术能够在抗生素等药物抑菌实验中快速、准确地检测细菌的生长状态和活力,为抗生素等药物抑菌机理研究中的检测技术提供数据支持,也有助于拓展流式细胞技术在细菌凋亡研究领域的应用。In response to the current shortcomings of quantitative indicators,inability to accurately evaluate the growth status of bacteria,and long experimental cycles in the antibacterial testing of antibiotic drugs,this study explores the application of flow cytometry technology in the antibacterial testing of Xanthomonas with different mass concentrations(0.02~20 mg/mL)and treatment time(1 and 2 hours).The results show that under the same treatment time,the higher the concentration of Shenqinmycin is,the more necrotic cells in the late stage of apoptosis.Under the same treatment concentration,the proportion of dead cells significantly increases with the increase of treatment time with Shenqinmycin.Flow cytometry can quickly and accurately detect the growth status and vitality of bacteria in antibacterial experiments of antibiotics and other drugs.The results may provide scientific data support for the core technology in detecting the antibacterial mechanism of antibiotics and other drugs,and also help to expand the application of flow cytometry in the field of bacterial apoptosis research.

关 键 词：流式细胞技术 抑菌检测 申嗪霉素 黄单胞菌 细菌凋亡 

分 类 号：G642[文化科学—高等教育学]