褪黑素对牛颗粒细胞凋亡相关基因表达的影响  

Effects of melatonin on expression of apoptosis-related genes in bovine granulosa cells

作　　者：郑梦浩 李瑞 刘彦彦 王佳琪 庞训胜[1] 刘文举 王淑娟[1] ZHENG Menghao;LI Rui;LIU Yanyan;WANG Jiaqi;PANG Xunsheng;LIU Wenju;WANG Shujuan(College of Animal Science,Anhui Science and Technology University,Fengyang 233100,China;College of Life and Health Science,Anhui Science and Technology University,Fengyang 233100,China)

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100 [2]安徽科技学院生命与健康科学学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2024年第3期11-18,共8页Journal of Anhui Science and Technology University

基　　金：安徽省自然科学基金(2008085MC94);安徽省重点研究与开发计划项目(202204c06020076);安徽省高校自然科学研究项目(KJ2021A0869)。

摘　　要：目的:探讨不同褪黑素浓度下处理不同时间对牛颗粒细胞凋亡相关基因表达的影响。方法:牛颗粒细胞从卵巢卵泡(直径3~8 mm)中分离并培养,培养3代后用于试验,分别在培养液中加入终浓度为0、10^(-3)、10^(-5)、10^(-7)、10^(-9)、10^(-11)mol/L褪黑素,培养24、48、72 h后,分别收集细胞,提取总RNA,利用实时定量PCR方法检测各组细胞中抗凋亡(BCL2和BCL-XL)、促凋亡(BAX、TP53和CASP3)基因表达情况。结果:不同剂量褪黑素作用24、48、72 h,可显著提高牛颗粒细胞中BCL2和BCL-XL的表达水平,抑制BAX、TP53、CASP3基因表达(P<0.05),但10^(-3)、10^(-5)mol/L组的BCL-XL表达量均无显著性变化(P>0.05),而72 h时,10^(-7)、10^(-9)、10^(-11)mol/L组TP53基因表达显著增加(P<0.05)。结论:不同浓度褪黑素处理不同时间对牛颗粒细胞中凋亡相关基因表达存在不同影响,褪黑素促进抗凋亡基因和抑制促凋亡基因的表达。Objective:To investigate the effects of different melatonin concentrations at different times on the expression of apoptosis-related genes in bovine granulosa cells.Methods:Bovine granulosa cells were isolated and cultured from bovine ovarian follicles(3-8 mm in diameter).Bovine granulosa cells were cultured for three generations and then entered the experiment.Melatonin of 0,10^(-3),10^(-5),10^(-7),10^(-9),10^(-11)mol/L was added to the culture medium.After 24,48,72 h of culture,the cells were collected and total RNA was extracted.The expressions of anti-apoptotic(BCL 2 and BCL-XL)and pro-apoptotic(BAX,TP 53 and CASP 3)genes in each group were detected by real-time PCR.Results:The effects of different doses of melatonin for 24,48,72 h significantly promoted the expression levels of BCL 2 and BCL-XL and inhibited the expression of BAX,TP 53 and CASP 3 genes in bovine granulosa cells(P<0.05),but there was no significant change in the expression of BCL-XL in both the 10^(-3),10^(-5)mol/L groups(P>0.05),whereas at 72 h,the 10^(-7),10^(-9),10^(-11)mol/L groups showed a significant increase in TP 53 gene expression at 72 h(P<0.05).Conclusion:Different concentrations and time of melatonin treatment have different effects on apoptosis-related genes in bovine granulosa cells.Melatonin promotes the expression of anti-apoptotic genes and inhibits the expression of pro-apoptotic genes.

关 键 词：褪黑素 牛 颗粒细胞 凋亡基因 

分 类 号：S823[农业科学—畜牧学]