离子液体辅助微波法提取橘皮中黄酮成分  

Extraction of flavonoids components from orange peel by ionic liquid-assisted microwave method

作　　者：闫平[1] 章思怡 严惠仲 张彦[1] YAN Ping;ZHANG Siyi;YAN Huizhong;ZHANG Yan(College of Pharmacy,Xi′an Medical University,Xi′an 710021,China)

机构地区：[1]西安医学院,陕西西安710021

出　　处：《化工科技》2024年第1期24-30,共7页Science & Technology in Chemical Industry

基　　金：国家自然科学基金青年项目(81903579);西安市科技局农业技术研发项目(21NYYF062);西安医学院2021年青年科研基金项目(2021QN23);西安医学院科研提升计划项目(2022NLTS082)。

摘　　要：优化离子液体辅助微波法提取橘皮中的黄酮工艺,为进一步提取橘皮中的黄酮类化合物提供依据。将离子液体加入提取溶剂中辅助微波法提取橘皮中的黄酮,依次改变离子液体的种类、浓度、提取溶剂总体积、药材质量比、微波时间、微波温度。以总黄酮提取率为指标,在确定单因素最佳条件基础上结合响应曲面设计优化最佳提取工艺。用HPLC法测定柚皮甙、橙皮苷、新橙皮苷等成分的含量,探究不同提取方法提取橘皮中黄酮的变化规律。最优提取条件为微波功率400 W,m(原料)∶V(提取液)=20 g/mL,V(乙醇)∶V(离子液体)=12,t=50℃,提取橘皮黄酮的提取率预测值为0.196 501%。比较传统方法和离子液体法黄酮中主要成分含量,后者提取率较高。The extraction of flavonoids from orange peel by ionic liquid assisted microwave was optimized to provide a basis for further extraction of flavonoids in orange peel.The ionic liquid was added to the extraction solvent to extract the flavonoids in the orange peel.The type,concentration,total volume of extraction solvent and mass ratio of medicinal materials,microwave time and microwave temperature were changed in turn.The extraction rate of total flavonoids was selected as the index,and the optimal extraction process was optimized based on the optimal single factor conditions and response surface model.The content of naringin,hesperidin,neohesperidin was detected by HPLC and the change pattern of flavonoids in orange peel by different extraction methods were explored.The optimal extraction conditions is microwave power 400 W,m(raw material)∶V(extract liquid)=20 g/mL,V(ethanol)∶V(ionic liquid)=12,temperature 50℃,the extraction ratio under the optimal process was 0.196501%.Comparing the content of main components in flavonoids using traditional methods and ionic liquid methods,the latter has a higher extraction rate.

关 键 词：离子液体 微波 橘皮 黄酮 

分 类 号：R284.1[医药卫生—中药学]