转PsnNAC007高耐旱性小黑杨种质创制及其特性分析  

Germplasm Innovation and Characteristic Analysis of Transgenic PsnNAC007 Populus simonii×P.nigra with High Drought Tolerance

作　　者：武晓倩 何旭[1] 高境烩 李爽[1] WU Xiaoqian;HE Xu;GAO Jinghui;LI Shuang(State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University),Harbin 150040)

机构地区：[1]林木遗传育种全国重点实验室(东北林业大学),哈尔滨150040

出　　处：《植物研究》2024年第3期349-360,共12页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(32001331)。

摘　　要：小黑杨(Populus simonii×P.nigra)是东北地区速生、耐寒、材性优良的树种。为了创制适种范围广、耐旱性状明显改良的林木新种质,利用小黑杨为材料,以干旱胁迫关键响应因子PsnNAC007转录因子为对象,创制了小黑杨过表达OE-PsnNAC007转基因植株。对OE-PsnNAC007转基因植株的生长指标、干旱胁迫适应能力、净光合速率(P_(n))、蒸腾速率(T_(r))、气孔导度(G_(s))、导水率指标、细胞形态和木材组分进行分析。结果显示:与野生型小黑杨相比,转基因植株的生长情况无明显差异,而干旱胁迫成活率提高了26.15%。在干旱胁迫条件下,转基因植株气孔导度减小、蒸腾速率下降、水分利用效率明显提高,植株茎干的导水率损失显著减少。解剖学分析发现,PsnNAC007的过量表达导致植株茎干产生更多、更小的导管细胞,这种细胞特性有利于植株在干旱条件下维持水分连续高效的运输。木材组分分析发现,转基因植株茎干木质素沉积明显增强,构成纤维素、半纤维素的单糖含量均无明显变化。Populus simonii×P.nigra is a fast-growing and cold-tolerant tree species with elite wood properties in northeast China.In order to produce a new forest germplasm with more widely planting and improved drought tolerance,this study successfully generated transgenic P.simonii×P.nigra using PsnNAC007,a key drought response transcription factor gene.Growth characteristics,drought tolerant ability,net photosynthetic rate(P_(n)),transpiration rate(T_(r)),stomatal conductance(G_(s)),water conductance,cell morphology and wood components of overexpressed PsnNAC007 transgenic plants were analyzed respectively.The results showed that there was no significant difference in growth between transgenic and wild-type plants,but drought survival rate was increased by 26.15%.Under drought condition,transgenic plants exhibited reduced Cleaf and T_(r),improved water use efficiency,and lower water conductance loss.Anatomical analysis showed that the overexpression of PsnNAC007 led to more and smaller stem xylem vessels,which were conducive to the continuous and efficient water transport in plants under drought conditions.Based on wood components analysis,lignin deposition in stems of transgenic plants was increased significantly,and monosaccharides that constituted cellulose and hemicellulose showed no significant change.

关 键 词：小黑杨 干旱胁迫 转录因子 遗传转化 

分 类 号：S792.11[农业科学—林木遗传育种]