花楸属复叶组植物倍性水平及基因组大小变异  

Ploidy Level and Genome Size Variation in Sorbus sect.Sorbus(Rosaceae)

作　　者：祁奇 侯文祥 邱靖[2] 耿礼阳 陈昕 QI Qi;HOU Wenxiang;QIU Jin;GENG Liyang;CHEN Xin(Co-innovation Center for Sustainable Forestry in Southern China,College of Biology and The Environment,Nanjing Forestry University,Nanjing 210037;Sanjiang University,Nanjing 210012)

机构地区：[1]南京林业大学南方现代林业协同创新中心,南京林业大学生物与环境学院,南京210037 [2]三江学院,南京210012

出　　处：《植物研究》2024年第3期441-447,共7页Bulletin of Botanical Research

基　　金：江苏省自然科学基金项目(BK20141472);2021年江苏省高校“青蓝工程”优秀青年骨干教师培养计划。

摘　　要：为探究花楸属复叶组(Sorbus sect.Sorbus)植物的倍性和基因组大小变异,以水稻品种日本晴(Oryza sativa subsp.japonica ‘Nipponbare’)为内标植物,应用流式细胞术对植物体细胞所含的DNA含量进行测定。结果表明:19种植物的基因组大小为0.648~0.803 pg,有二、三、四倍体3种倍性水平,其中二倍体植物有15种,2C值为1.335~1.607 pg;三倍体仅喜马拉雅花楸(S.himalaica)1种,2C值为2.137 pg;白毛花楸(S.albopilosa)、西康花楸(S.prattii)和假川滇花楸(S.pseudovilmorinii)为四倍体,2C值分别为2.594、2.891、2.751 pg。首次报道了16种花楸属植物的2C值,并报道了5种植物新的倍性水平,表明花楸属植物在种间及种内均存在倍性变化。In order to explore the ploidy level and genome size of Sorbus sect.Sorbus species,the nuclear DNA content from somatic cells was measured by flow cytometry and Oryza sativa subsp.japonica ‘Nipponbare' was used as the internal control.The results showed that the genome size of 19 species ranged from 0.648 to 0.803 pg,with the diploid,triploid and tetraploid levels,including 15 diploid species with 2C values ranging from 1.335 to 1.607 pg for,and one triploid species S.himalaica with 2C values of 2.137 pg,and three tetraploid species,S.albopilosa,S.prattii and S.pseudovilmorinii,with 2C values of 2.594,2.891 and 2.751 pg,respectively.The 2C values of 16 species and new ploidy levels of five species were reported for the first time,indicating that there were changes of ploidy heterogeneity among interspecific and intraspecific in Sorbus sect.Sorbus.

关 键 词：基因组大小 倍性 流式细胞术 花楸属复叶组 蔷薇科 

分 类 号：S792.25[农业科学—林木遗传育种]